7VWZ
Cryo-EM structure of Rob-dependent transcription activation complex in a unique conformation
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150804.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (F) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli K-12 | Sigma-70 |
| 6 | G (1) | micF promoter DNA forward strand | polymer | 70 | 21574.9 | 1 | Escherichia coli | ||
| 7 | H (2) | micF promoter DNA reverse strand | polymer | 70 | 21751.9 | 1 | Escherichia coli | ||
| 8 | I, J (G, I) | Right origin-binding protein | polymer | 289 | 33184.8 | 2 | UniProt (P0ACI0) Pfam (PF12833) Pfam (PF06445) | Escherichia coli K-12 | |
| 9 | K, L (D) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 10 | M (D) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
C: 1 - 1342 (UniProt: P0A8V2)
| PDB | External Database | Details |
|---|---|---|
| Val 516 | Asp 516 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 569587.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 569742.5 |
