7VWN

The structure of an engineered PET hydrolase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Poly(ethylene terephthalate) hydrolase	polymer	270	28515.6	1	UniProt (A0A0K8P6T7) Pfam (PF01738) In PDB	Ideonella sakaiensis (strain NBRC 110686 / TISTR 2288 / 201-F6)	PET hydrolase,PETase,PET-digesting enzyme
2	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
3		water	water		18.0	282	Chemie (HOH)

Sequence modifications

A: 29 - 290 (UniProt: A0A0K8P6T7)

PDB	External Database	Details
Ser 29	Thr 29	engineered mutation
Asn 95	Lys 95	engineered mutation
Arg 168	Ile 168	engineered mutation
Val 181	Pro 181	engineered mutation
Val 214	Ser 214	engineered mutation
Cys 233	Asn 233	engineered mutation
Asp 248	Ala 248	engineered mutation
Ala 280	Arg 280	engineered mutation
Cys 282	Ser 282	engineered mutation
Leu 291	-	expression tag
Glu 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28515.6
Non-Polymers*	Number of molecules	3
	Total formula weight	106.4
All*	Total formula weight	28622.0

*Water molecules are not included.