7VWD

Crystal Structure of the Y53F/N55A mutant of LEH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Limonene-1,2-epoxide hydrolase	polymer	155	17304.4	4	UniProt (Q9ZAG3) Pfam (PF07858)	Rhodococcus erythropolis
2	E, L (A, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	F, I (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
4	G, H, J, K, M (A, B, C)	SODIUM ION	non-polymer		23.0	5	Chemie (NA)
5	N (D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6	O, P, Q, R (A, B, C, D)	water	water		18.0	179	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 149 (UniProt: Q9ZAG3)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Phe 53	Tyr 53	engineered mutation
Ala 55	Asn 55	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	69217.7
Non-Polymers*	Number of molecules	10
	Total formula weight	529.4
All*	Total formula weight	69747.1

*Water molecules are not included.