7VVJ

PTHrP-bound human PTH1R in complex with Gs

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44255.7	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Norway rat)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7547.7	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (cattle)	G gamma-I
4	N	nanobody Nb35	polymer	137	15015.7	1		unidentified
5	P	PTHrP[1-36]	polymer	36	4269.9	1	UniProt (P12272) Pfam (PF01279) In PDB	Homo sapiens
6	R	Parathyroid hormone/parathyroid hormone-related peptide receptor	polymer	482	55194.3	1	UniProt (Q03431) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (human)	PTH/PTHrP type I receptor,PTH/PTHr receptor,Parathyroid hormone 1 receptor,PTH1 receptor

Sequence modifications

A: 5 - 384 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation
Tyr 63	Leu 63	engineered mutation
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 362	Ile 372	engineered mutation
Ile 365	Val 375	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

G: 1 - 67 (UniProt: P63212)

PDB	External Database	Details
Ser 68	-	expression tag

R: 27 - 501 (UniProt: Q03431)

PDB	External Database	Details
Asp 20	-	expression tag
Tyr 21	-	expression tag
Lys 22	-	expression tag
Asp 23	-	expression tag
Asp 24	-	expression tag
Asp 25	-	expression tag
Asp 26	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	165027.7
All*	Total formula weight	165027.7

*Water molecules are not included.