7VUI

Cryo-EM structure of a class A orphan GPCR

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	261	30422.2	1	UniProt (P63092-2) Pfam (PF00503) UniProt (by SIFTS) (P63092) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38045.6	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	N	Nanobody 35	polymer	157	17057.3	1		Homo sapiens
5	R	Chimera of Endo-1,4-beta-xylanase and Probable G-protein coupled receptor 139	polymer	533	60890.1	1	UniProt (P09850) UniProt (Q6DWJ6) Pfam (PF00457) Pfam (PF00001) In PDB	Bacillus circulans	Xylanase,1,4-beta-D-xylan xylanohydrolase,G(q)-coupled orphan receptor GPRg1,G-protein-coupled receptor PGR3
6	A	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
7	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	R	3-chloranyl-N-[2-oxidanylidene-2-[[(1S)-1-phenylethyl]amino]ethyl]benzamide	non-polymer		316.8	1	Chemie (7ZQ)

Sequence modifications

A: 5 - 195 (UniProt: P63092-2)

PDB	External Database	Details
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Glu -1	-	expression tag
Asn 0	-	expression tag
Leu 1	-	expression tag
Tyr 2	-	expression tag
Phe 3	-	expression tag
Gln 4	-	expression tag
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation

A: 204 - 361 (UniProt: P63092-2)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 235	engineered mutation
Asp 252	Ser 238	engineered mutation
-	Asn 240	deletion
-	Met 241	deletion
-	Val 242	deletion
-	Ile 243	deletion
-	Arg 244	deletion
-	Glu 245	deletion
-	Asp 246	deletion
-	Asn 247	deletion
-	Gln 248	deletion
-	Thr 249	deletion
Ala 362	-	expression tag
Arg 363	-	expression tag
Arg 364	-	expression tag
Ile 365	-	expression tag
Phe 366	-	expression tag
Asn 367	-	expression tag
Asp 368	-	expression tag
Cys 369	-	expression tag
Lys 370	-	expression tag
Asp 371	-	expression tag
Ala 372	-	expression tag
Ile 373	-	expression tag
Leu 374	-	expression tag
Gln 375	-	expression tag
Met 376	-	expression tag
Asn 377	-	expression tag
Leu 378	-	expression tag
Arg 379	-	expression tag
Glu 380	-	expression tag
Tyr 381	-	expression tag
Asn 382	-	expression tag
Leu 383	-	expression tag
Val 384	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Ile -5	-	expression tag
Gly -4	-	expression tag
Arg -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Phe 1	-	expression tag

R: -183 - 1 (UniProt: P09850)

PDB	External Database	Details
His -211	-	expression tag
His -210	-	expression tag
His -209	-	expression tag
His -208	-	expression tag
His -207	-	expression tag
His -206	-	expression tag
His -205	-	expression tag
His -204	-	expression tag
His -203	-	expression tag
His -202	-	expression tag
Asp -201	-	expression tag
Tyr -200	-	expression tag
Lys -199	-	expression tag
Asp -198	-	expression tag
Asp -197	-	expression tag
Asp -196	-	expression tag
Asp -195	-	expression tag
Lys -194	-	expression tag
Glu -193	-	expression tag
Asn -192	-	expression tag
Leu -191	-	expression tag
Tyr -190	-	expression tag
Phe -189	-	expression tag
Gln -188	-	expression tag
Ser -187	-	expression tag
Gly -186	-	expression tag
Ala -185	-	expression tag
Pro -184	-	expression tag
Phe -173	Asp 39	engineered mutation
Asp -62	Arg 150	engineered mutation

R: 2 - 321 (UniProt: Q6DWJ6)

PDB	External Database	Details
Val 62	Ser 62	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	154276.4
Non-Polymers*	Number of molecules	3
	Total formula weight	864.3
All*	Total formula weight	155140.7

*Water molecules are not included.