7VUI
Cryo-EM structure of a class A orphan GPCR
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 261 | 30422.2 | 1 | UniProt (P63092-2) Pfam (PF00503) UniProt (by SIFTS) (P63092) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein,Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 346 | 38045.6 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (Human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (Human) | G gamma-I |
| 4 | D (N) | Nanobody 35 | polymer | 157 | 17057.3 | 1 | Homo sapiens | ||
| 5 | E (R) | Chimera of Endo-1,4-beta-xylanase and Probable G-protein coupled receptor 139 | polymer | 533 | 60890.1 | 1 | UniProt (P09850) UniProt (Q6DWJ6) Pfam (PF00001) | Bacillus circulans | Xylanase,1,4-beta-D-xylan xylanohydrolase,G(q)-coupled orphan receptor GPRg1,G-protein-coupled receptor PGR3 |
| 6 | F (A) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) PubChem (177679032) PubChem (135400627) PubChem (135487750) PubChem (136088935) PubChem (136803486) PubChem (135398633) PubChem (135544356) PubChem (135547949) PubChem (135600366) PubChem (135749822) PubChem (136005188) PubChem (136073923) PubChem (136240914) PubChem (136664747) PubChem (136772743) PubChem (136772744) PubChem (136803487) PubChem (137286749) PubChem (164715984) PubChem (171699466) | |||
| 7 | G (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 8 | H (R) | 3-chloranyl-N-[2-oxidanylidene-2-[[(1S)-1-phenylethyl]amino]ethyl]benzamide | non-polymer | 316.8 | 1 | Chemie (7ZQ) PubChem (2548543) PubChem (2548547) PubChem (4879329) |
Sequence modifications
A: 5 - 195 (UniProt: P63092-2)
A: 204 - 361 (UniProt: P63092-2)
B: 2 - 340 (UniProt: P62873)
R: -183 - 1 (UniProt: P09850)
R: 2 - 321 (UniProt: Q6DWJ6)
| PDB | External Database | Details |
|---|---|---|
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Leu 1 | - | expression tag |
| Tyr 2 | - | expression tag |
| Phe 3 | - | expression tag |
| Gln 4 | - | expression tag |
| Asp 49 | Gly 49 | engineered mutation |
| Asn 50 | Glu 50 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 196 | - | linker |
| Gly 197 | - | linker |
| Ser 198 | - | linker |
| Gly 199 | - | linker |
| Gly 200 | - | linker |
| Ser 201 | - | linker |
| Gly 202 | - | linker |
| Gly 203 | - | linker |
| Asp 249 | Ala 235 | engineered mutation |
| Asp 252 | Ser 238 | engineered mutation |
| - | Asn 240 | deletion |
| - | Met 241 | deletion |
| - | Val 242 | deletion |
| - | Ile 243 | deletion |
| - | Arg 244 | deletion |
| - | Glu 245 | deletion |
| - | Asp 246 | deletion |
| - | Asn 247 | deletion |
| - | Gln 248 | deletion |
| - | Thr 249 | deletion |
| Ala 362 | - | expression tag |
| Arg 363 | - | expression tag |
| Arg 364 | - | expression tag |
| Ile 365 | - | expression tag |
| Phe 366 | - | expression tag |
| Asn 367 | - | expression tag |
| Asp 368 | - | expression tag |
| Cys 369 | - | expression tag |
| Lys 370 | - | expression tag |
| Asp 371 | - | expression tag |
| Ala 372 | - | expression tag |
| Ile 373 | - | expression tag |
| Leu 374 | - | expression tag |
| Gln 375 | - | expression tag |
| Met 376 | - | expression tag |
| Asn 377 | - | expression tag |
| Leu 378 | - | expression tag |
| Arg 379 | - | expression tag |
| Glu 380 | - | expression tag |
| Tyr 381 | - | expression tag |
| Asn 382 | - | expression tag |
| Leu 383 | - | expression tag |
| Val 384 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ile -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Arg -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Phe 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His -211 | - | expression tag |
| His -210 | - | expression tag |
| His -209 | - | expression tag |
| His -208 | - | expression tag |
| His -207 | - | expression tag |
| His -206 | - | expression tag |
| His -205 | - | expression tag |
| His -204 | - | expression tag |
| His -203 | - | expression tag |
| His -202 | - | expression tag |
| Asp -201 | - | expression tag |
| Tyr -200 | - | expression tag |
| Lys -199 | - | expression tag |
| Asp -198 | - | expression tag |
| Asp -197 | - | expression tag |
| Asp -196 | - | expression tag |
| Asp -195 | - | expression tag |
| Lys -194 | - | expression tag |
| Glu -193 | - | expression tag |
| Asn -192 | - | expression tag |
| Leu -191 | - | expression tag |
| Tyr -190 | - | expression tag |
| Phe -189 | - | expression tag |
| Gln -188 | - | expression tag |
| Ser -187 | - | expression tag |
| Gly -186 | - | expression tag |
| Ala -185 | - | expression tag |
| Pro -184 | - | expression tag |
| Phe -173 | Asp 39 | engineered mutation |
| Asp -62 | Arg 150 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Val 62 | Ser 62 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 154276.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 864.3 | |
| All* | Total formula weight | 155140.7 |
