7VTI
Crystal structure of the Cas13bt3-crRNA binary complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cas13bt3 | polymer | 777 | 91061.5 | 1 | UniProt (A0A660UUL5) | Planctomycetes bacterium | |
| 2 | B (B) | crRNA | polymer | 41 | 13206.8 | 1 | synthetic construct | ||
| 3 | C, D, E, F, G (A) | CHLORIDE ION | non-polymer | 35.5 | 5 | Chemie (CL) | |||
| 4 | EA, FA, GA, H, I... (B, A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 22 | Chemie (EDO) | |||
| 5 | AA (A) | BROMIDE ION | non-polymer | 79.9 | 1 | Chemie (BR) | |||
| 6 | BA, CA, DA (B) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 7 | HA, IA (A, B) | water | water | 18.0 | 339 | Chemie (HOH) |
Sequence modifications
A: 1 - 775 (UniProt: A0A660UUL5)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ala 84 | Arg 84 | engineered mutation |
| Ala 89 | His 89 | engineered mutation |
| Ala 739 | Arg 739 | engineered mutation |
| Ala 744 | His 744 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 104268.4 | |
| Non-Polymers* | Number of molecules | 31 |
| Total formula weight | 1695.6 | |
| All* | Total formula weight | 105963.9 |
