7VT4

Crystal structure of mutant E393Q of MtGlu5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Endoglucanase H	polymer	468	52976.1	2	UniProt (A0A399DY85) Pfam (PF00150)	Meiothermus taiwanensis WR-220
2	C, D, E, M, N... (A, B)	GLYCEROL	non-polymer		92.1	6	Chemie (GOL)
3	F, G, H, I, J... (A, B)	SULFATE ION	non-polymer		96.1	13	Chemie (SO4)
4	V, W (A, B)	water	water		18.0	1540	Chemie (HOH)

Sequence modifications

A, B: 2 - 455 (UniProt: A0A399DY85)

PDB	External Database	Details
Met 1	-	initiating methionine
Gln 393	Glu 408	engineered mutation
Lys 456	-	expression tag
Leu 457	-	expression tag
Ala 458	-	expression tag
Ala 459	-	expression tag
Ala 460	-	expression tag
Leu 461	-	expression tag
Glu 462	-	expression tag
His 463	-	expression tag
His 464	-	expression tag
His 465	-	expression tag
His 466	-	expression tag
His 467	-	expression tag
His 468	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	105952.3
Non-Polymers*	Number of molecules	19
	Total formula weight	1801.4
All*	Total formula weight	107753.6

*Water molecules are not included.