7VN8
Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning GTCACGTGAC
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | C, D, A, B | Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1 | polymer | 439 | 48419.8 | 4 | UniProt (A0A4P1LXE0) UniProt (Q8S307) Pfam (PF01547) Pfam (PF05687) UniProt (by SIFTS) (P0AEX9) In PDB | Serratia sp. (strain FS14) | Protein BIN2 SUBSTRATE 2 |
2 | G, H, E, F | DNA (5'-D(*TP*TP*GP*TP*CP*AP*CP*GP*TP*GP*AP*CP*AP*AP*A)-3') | polymer | 15 | 4593.0 | 4 | synthetic construct | ||
3 | K, L, M, N | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 4 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | |||
4 | C, D, G, H, A... | 1,2-ETHANEDIOL | non-polymer | 62.1 | 11 | Chemie (EDO) | |||
5 | water | water | 18.0 | 231 | Chemie (HOH) |
Sequence modifications
C, D, A, B: -347 - 20 (UniProt: A0A4P1LXE0)
C, D, A, B: 21 - 90 (UniProt: Q8S307)
PDB | External Database | Details |
---|---|---|
Met -348 | - | initiating methionine |
Ala -266 | Asp 84 | engineered mutation |
Ala -265 | Lys 85 | engineered mutation |
Ala -176 | Glu 174 | engineered mutation |
Ala -175 | Asn 175 | engineered mutation |
Ala -109 | Lys 241 | engineered mutation |
Ala 11 | Glu 361 | engineered mutation |
Ala 14 | Lys 364 | engineered mutation |
Ala 15 | Asp 365 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 212051.4 | |
Branched | Number of molecules | 4 |
Total formula weight | 1369.2 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 682.7 | |
All* | Total formula weight | 214103.3 |