7VN2

Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning ATCACGTGAT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (C, A)	Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1	polymer	439	48419.8	2	UniProt (A0A4P1LXE0) UniProt (Q8S307) Pfam (PF01547) Pfam (PF05687) UniProt (by SIFTS) (P0AEX9)	Serratia sp. (strain FS14)	Protein BIN2 SUBSTRATE 2
2	B, D (G, E)	DNA (5'-D(*TP*TP*AP*TP*CP*AP*CP*GP*TP*GP*AP*TP*AP*AP*A)-3')	polymer	15	4592.0	2		synthetic construct
3	E, F (D, F)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)
4	G, H, I, J (C, A)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
5	K, L, M, N (C, G, A, E)	water	water		18.0	43	Chemie (HOH)

Sequence modifications

C, A: -347 - 20 (UniProt: A0A4P1LXE0)

PDB	External Database	Details
Met -348	-	initiating methionine
Ala -266	Asp 84	engineered mutation
Ala -265	Lys 85	engineered mutation
Ala -176	Glu 174	engineered mutation
Ala -175	Asn 175	engineered mutation
Ala -109	Lys 241	engineered mutation
Ala 11	Glu 361	engineered mutation
Ala 14	Lys 364	engineered mutation
Ala 15	Asp 365	engineered mutation

C, A: 21 - 90 (UniProt: Q8S307)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	106023.7
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	4
	Total formula weight	248.3
All*	Total formula weight	106956.6

*Water molecules are not included.