7VKT
cryo-EM structure of LTB4-bound BLT1 in complex with Gi protein
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Leukotriene B4 receptor 1 | polymer | 352 | 37740.5 | 1 | UniProt (Q15722) Pfam (PF00001) | Homo sapiens (human) | LTB4-R 1,LTB4-R1,Chemoattractant receptor-like 1,G-protein coupled receptor 16,P2Y purinoceptor 7,P2Y7 |
| 2 | B (B) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (D) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (E) | scFv16 | polymer | 247 | 26277.3 | 1 | Homo sapiens (Human) | ||
| 6 | F (A) | LEUKOTRIENE B4 | non-polymer | 336.5 | 1 | Chemie (LTB) | |||
| 7 | G, H, I, J (A) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 4 | Chemie (Y01) | |||
| 8 | K (A) | [(2R)-2-[(Z)-hexadec-9-enoyl]oxy-3-[oxidanyl-[(2S,3R,5R,6S)-2,3,4,5,6-pentakis(oxidanyl)cyclohexyl]oxy-phosphoryl]oxy-propyl] octadecanoate | non-polymer | 837.1 | 1 | Chemie (8IO) | |||
| 9 | L (A) | water | water | 18.0 | 7 | Chemie (HOH) |
Sequence modifications
A: 1 - 352 (UniProt: Q15722)
B: 1 - 354 (UniProt: P63096)
C: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Trp 106 | Leu 106 | engineered mutation |
| Ile 196 | Ala 196 | engineered mutation |
| Phe 287 | Cys 287 | engineered mutation |
| Ala 310 | Ser 310 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 150208.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 3120.4 | |
| All* | Total formula weight | 153328.9 |
