7VIH
Cryo-EM structure of Gi coupled Sphingosine 1-phosphate receptor bound with CBP-307
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 357 | 39286.9 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 2 | B (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 3 | C (E) | scFv16 | polymer | 251 | 26679.7 | 1 | Mus musculus | ||
| 4 | D (F) | Sphingosine 1-phosphate receptor 1 | polymer | 394 | 44483.4 | 1 | UniProt (P21453) Pfam (PF00001) | Homo sapiens (human) | S1P receptor 1,S1P1,Endothelial differentiation G-protein coupled receptor 1,Sphingosine 1-phosphate receptor Edg-1,S1P receptor Edg-1 |
| 5 | E (D) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 6 | F (F) | 1-[[2-fluoranyl-4-[5-[4-(2-methylpropyl)phenyl]-1,2,4-oxadiazol-3-yl]phenyl]methyl]azetidine-3-carboxylic acid | non-polymer | 409.5 | 1 | Chemie (7I4) |
Sequence modifications
A: 1 - 339 (UniProt: P62873)
F: 1 - 382 (UniProt: P21453)
| PDB | External Database | Details |
|---|---|---|
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| Leu -11 | - | expression tag |
| Glu -10 | - | expression tag |
| Val -9 | - | expression tag |
| Leu -8 | - | expression tag |
| Phe -7 | - | expression tag |
| Gln -6 | - | expression tag |
| Gly -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Leu 383 | - | expression tag |
| Glu 384 | - | expression tag |
| His 385 | - | expression tag |
| His 386 | - | expression tag |
| His 387 | - | expression tag |
| His 388 | - | expression tag |
| His 389 | - | expression tag |
| His 390 | - | expression tag |
| His 391 | - | expression tag |
| His 392 | - | expression tag |
| His 393 | - | expression tag |
| His 394 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 158726.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 409.5 | |
| All* | Total formula weight | 159135.7 |
