7VH1

Cryo-EM structure of Machupo virus dimeric L-Z complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	RNA-directed RNA polymerase L	polymer	2238	253456.3	1	UniProt (Q6IUF8) Pfam (PF17296) Pfam (PF06317) In PDB	Machupo virus (MACV)	Protein L,Large structural protein,Replicase,Transcriptase
2	B	Maltose/maltodextrin-binding periplasmic protein,RING finger protein Z	polymer	496	54913.9	1	UniProt (P0AEX9) UniProt (Q6IUF9) Pfam (PF01547) Pfam (PF03854) In PDB	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Protein Z,Zinc-binding protein
3	B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 2209 (UniProt: Q6IUF8)

PDB	External Database	Details
Trp 2210	-	expression tag
Ser 2211	-	expression tag
His 2212	-	expression tag
Pro 2213	-	expression tag
Gln 2214	-	expression tag
Phe 2215	-	expression tag
Glu 2216	-	expression tag
Lys 2217	-	expression tag
Gly 2218	-	expression tag
Gly 2219	-	expression tag
Gly 2220	-	expression tag
Ser 2221	-	expression tag
Gly 2222	-	expression tag
Gly 2223	-	expression tag
Gly 2224	-	expression tag
Ser 2225	-	expression tag
Gly 2226	-	expression tag
Gly 2227	-	expression tag
Ser 2228	-	expression tag
Ser 2229	-	expression tag
Ala 2230	-	expression tag
Trp 2231	-	expression tag
Ser 2232	-	expression tag
His 2233	-	expression tag
Pro 2234	-	expression tag
Gln 2235	-	expression tag
Phe 2236	-	expression tag
Glu 2237	-	expression tag
Lys 2238	-	expression tag

B: -392 - -27 (UniProt: P0AEX9)

PDB	External Database	Details
Met -401	-	expression tag
His -400	-	expression tag
His -399	-	expression tag
His -398	-	expression tag
His -397	-	expression tag
His -396	-	expression tag
His -395	-	expression tag
His -394	-	expression tag
His -393	-	expression tag
Asn -26	-	linker
Ser -25	-	linker
Ser -24	-	linker
Ser -23	-	linker
Asn -22	-	linker
Asn -21	-	linker
Asn -20	-	linker
Asn -19	-	linker
Asn -18	-	linker
Asn -17	-	linker
Asn -16	-	linker
Asn -15	-	linker
Asn -14	-	linker
Asn -13	-	linker
Leu -12	-	linker
Gly -11	-	linker
Ile -10	-	linker
Glu -9	-	linker
Leu -8	-	linker
Glu -7	-	linker
Val -6	-	linker
Leu -5	-	linker
Phe -4	-	linker
Gln -3	-	linker
Gly -2	-	linker
Pro -1	-	linker
Gly 0	-	linker
Ser 1	-	linker

B: 2 - 94 (UniProt: Q6IUF9)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	308370.2
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	308501.0

*Water molecules are not included.