7VF9

Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA-directed RNA polymerase subunit alpha	polymer	345	38264.3	2	UniProt (O52760) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118)	Pseudomonas aeruginosa PAO1	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1359	151225.3	1	UniProt (Q51561) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562)	Pseudomonas aeruginosa PAO1	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1412	156038.5	1	UniProt (Q9HWC9) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Pseudomonas aeruginosa PAO1	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	88	9783.9	1	UniProt (Q9HTM1) Pfam (PF01192)	Pseudomonas aeruginosa PAO1	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase sigma factor RpoS	polymer	338	38606.7	1	UniProt (P45684) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Pseudomonas aeruginosa PAO1	Sigma S,Sigma-38
6	G, H (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	I (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

A, B: 1 - 333 (UniProt: O52760)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

C: 1 - 1357 (UniProt: Q51561)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag

D: 2 - 1399 (UniProt: Q9HWC9)

PDB	External Database	Details
Met 0	-	initiating methionine
Leu 1	-	expression tag
Gly 1400	-	expression tag
Ser 1401	-	expression tag
Gly 1402	-	expression tag
Ser 1403	-	expression tag
Trp 1404	-	expression tag
Ser 1405	-	expression tag
His 1406	-	expression tag
Pro 1407	-	expression tag
Gln 1408	-	expression tag
Phe 1409	-	expression tag
Glu 1410	-	expression tag
Lys 1411	-	expression tag

F: 1 - 334 (UniProt: P45684)

PDB	External Database	Details
Gly -3	-	expression tag
Ala -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	432182.9
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	432338.1

*Water molecules are not included.