7VE7
Crystal structure of KRED mutant-F147L/L153Q/Y190P/L199A/M205F/M206F
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | 3-alpha-(Or 20-beta)-hydroxysteroid dehydrogenase | polymer | 253 | 26773.2 | 4 | UniProt (Q6WVP7) Pfam (PF13561) | Lactobacillus kefiri | NADPH dependent R-specific alcohol dehydrogenase,SDR family NAD(P)-dependent oxidoreductase |
| 2 | E, I (A, D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 3 | F, G, H, J (A, B, C, D) | NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 743.4 | 4 | Chemie (NAP) | |||
| 4 | K, L, M, N (A, B, C, D) | water | water | 18.0 | 372 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 252 (UniProt: Q6WVP7)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Leu 147 | Phe 147 | engineered mutation |
| Gln 153 | Leu 153 | engineered mutation |
| Pro 190 | Tyr 190 | engineered mutation |
| Ala 199 | Leu 199 | engineered mutation |
| Phe 205 | Met 205 | engineered mutation |
| Phe 206 | Met 206 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 107092.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 3022.2 | |
| All* | Total formula weight | 110114.9 |
