7VBI
Cryo-EM structure of the non-acylated tirzepatide (LY3298176)-bound human GLP-1R-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Isoform 3 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 357 | 41708.3 | 1 | UniProt (P63092-3) Pfam (PF00503) UniProt (by SIFTS) (P63092) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (N) | Nanobody 35 | polymer | 140 | 15343.0 | 1 | Escherichia coli | ||
| 3 | C (P) | Non-acylated_tirzepatide | polymer | 29 | 3237.7 | 1 | Homo sapiens | ||
| 4 | D (R) | Glucagon-like peptide 1 receptor | polymer | 440 | 50860.8 | 1 | UniProt (P43220) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (Human) | GLP-1 receptor,GLP-1-R,GLP-1R |
| 5 | E (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Rat) | Transducin beta chain 1 |
| 6 | F (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G gamma-I |
Sequence modifications
A: 12 - 394 (UniProt: P63092-3)
B: 2 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | conflict |
| Ala 226 | Gly 211 | conflict |
| - | Ser 237 | deletion |
| - | Tyr 238 | deletion |
| - | Asn 239 | deletion |
| - | Met 240 | deletion |
| - | Val 241 | deletion |
| - | Ile 242 | deletion |
| - | Arg 243 | deletion |
| - | Glu 244 | deletion |
| - | Asp 245 | deletion |
| - | Asn 246 | deletion |
| - | Gln 247 | deletion |
| Ala 268 | Glu 253 | conflict |
| Lys 271 | Asn 256 | conflict |
| Asp 274 | Lys 259 | conflict |
| Lys 280 | Arg 265 | conflict |
| Asp 284 | Thr 269 | conflict |
| Thr 285 | Ile 270 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 156795.2 | |
| All* | Total formula weight | 156795.2 |
