7VBI

Cryo-EM structure of the non-acylated tirzepatide (LY3298176)-bound human GLP-1R-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Isoform 3 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	357	41708.3	1	UniProt (P63092-3) Pfam (PF00503) UniProt (by SIFTS) (P63092) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	N	Nanobody 35	polymer	140	15343.0	1		Escherichia coli
3	P	Non-acylated_tirzepatide	polymer	29	3237.7	1		Homo sapiens
4	R	Glucagon-like peptide 1 receptor	polymer	440	50860.8	1	UniProt (P43220) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	GLP-1 receptor,GLP-1-R,GLP-1R
5	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Rat)	Transducin beta chain 1
6	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (Bovine)	G gamma-I

Sequence modifications

A: 12 - 394 (UniProt: P63092-3)

PDB	External Database	Details
Asn 54	Ser 54	conflict
Ala 226	Gly 211	conflict
-	Ser 237	deletion
-	Tyr 238	deletion
-	Asn 239	deletion
-	Met 240	deletion
-	Val 241	deletion
-	Ile 242	deletion
-	Arg 243	deletion
-	Glu 244	deletion
-	Asp 245	deletion
-	Asn 246	deletion
-	Gln 247	deletion
Ala 268	Glu 253	conflict
Lys 271	Asn 256	conflict
Asp 274	Lys 259	conflict
Lys 280	Arg 265	conflict
Asp 284	Thr 269	conflict
Thr 285	Ile 270	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	156795.2
All*	Total formula weight	156795.2

*Water molecules are not included.