7V7Z

Cryo-EM structure of SARS-CoV-2 S-Beta variant (B.1.351) in complex with Angiotensin-converting enzyme 2 (ACE2) ectodomain, three ACE2-bound form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Spike glycoprotein	polymer	1280	141830.9	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	S glycoprotein,E2,Peplomer protein
2	D, E, F (D, E, F)	Angiotensin-converting enzyme 2,Green fluorescent protein	polymer	861	98273.5	3	UniProt (Q9BYF1) Pfam (PF01401) Pfam (PF01353) UniProt (by SIFTS) (A0A059PIQ0)	Homo sapiens (Human)	Angiotensin-converting enzyme homolog,ACEH,Angiotensin-converting enzyme-related carboxypeptidase,ACE-related carboxypeptidase,Metalloprotease MPROT15
3	AA, BA, CA, DA, EA... (a, b, c, d, e...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	31	In PDB GlyTouCan (G42666HT)
4	AB, BB, LA, MA, NA... (C, D, A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	17	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 1205 (UniProt: P0DTC2)

PDB	External Database	Details
Phe 18	Leu 18	variant
Ala 80	Asp 80	variant
Gly 215	Asp 215	variant
-	Leu 241	deletion
-	Leu 242	deletion
-	Ala 243	deletion
Ile 243	Arg 246	engineered mutation
Asn 414	Lys 417	variant
Lys 481	Glu 484	variant
Tyr 498	Asn 501	variant
Gly 611	Asp 614	variant
Gly 679	Arg 682	engineered mutation
Ser 680	Arg 683	engineered mutation
Ser 682	Arg 685	engineered mutation
Val 698	Ala 701	variant
Pro 983	Lys 986	engineered mutation
Pro 984	Val 987	engineered mutation
Glu 1206	-	expression tag
Phe 1207	-	expression tag
Gly 1208	-	expression tag
Ser 1209	-	expression tag
Gly 1210	-	expression tag
Gly 1211	-	expression tag
Tyr 1212	-	expression tag
Ile 1213	-	expression tag
Pro 1214	-	expression tag
Glu 1215	-	expression tag
Ala 1216	-	expression tag
Pro 1217	-	expression tag
Arg 1218	-	expression tag
Asp 1219	-	expression tag
Gly 1220	-	expression tag
Gln 1221	-	expression tag
Ala 1222	-	expression tag
Tyr 1223	-	expression tag
Val 1224	-	expression tag
Arg 1225	-	expression tag
Lys 1226	-	expression tag
Asp 1227	-	expression tag
Gly 1228	-	expression tag
Glu 1229	-	expression tag
Trp 1230	-	expression tag
Val 1231	-	expression tag
Leu 1232	-	expression tag
Leu 1233	-	expression tag
Ser 1234	-	expression tag
Thr 1235	-	expression tag
Phe 1236	-	expression tag
Leu 1237	-	expression tag
Lys 1238	-	expression tag
Gly 1239	-	expression tag
Gln 1240	-	expression tag
Asp 1241	-	expression tag
Asn 1242	-	expression tag
Ser 1243	-	expression tag
Ala 1244	-	expression tag
Asp 1245	-	expression tag
Ile 1246	-	expression tag
Gln 1247	-	expression tag
His 1248	-	expression tag
Ser 1249	-	expression tag
Gly 1250	-	expression tag
Arg 1251	-	expression tag
Pro 1252	-	expression tag
Leu 1253	-	expression tag
Glu 1254	-	expression tag
Ser 1255	-	expression tag
Arg 1256	-	expression tag
Gly 1257	-	expression tag
Pro 1258	-	expression tag
Phe 1259	-	expression tag
Glu 1260	-	expression tag
Gln 1261	-	expression tag
Lys 1262	-	expression tag
Leu 1263	-	expression tag
Ile 1264	-	expression tag
Ser 1265	-	expression tag
Glu 1266	-	expression tag
Glu 1267	-	expression tag
Asp 1268	-	expression tag
Leu 1269	-	expression tag
Asn 1270	-	expression tag
Met 1271	-	expression tag
His 1272	-	expression tag
Thr 1273	-	expression tag
Gly 1274	-	expression tag
His 1275	-	expression tag
His 1276	-	expression tag
His 1277	-	expression tag
His 1278	-	expression tag
His 1279	-	expression tag
His 1280	-	expression tag

D, E, F: 1 - 615 (UniProt: Q9BYF1)
D, E, F: 616 - 861 (PDB: 7V7Z)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	720313.2
Branched	Number of molecules	31
	Total formula weight	13156.4
Non-Polymers*	Number of molecules	17
	Total formula weight	3760.5
All*	Total formula weight	737230.2

*Water molecules are not included.