7V6Z

Cryo-EM structure of Patched1 (V1084A mutant) in lipid nanodisc, 3.64 angstrom (reprocessed with the dataset of 7dzp)

Replaces:  7DZP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein patched homolog 1,Protein patched homolog 1	polymer	1090	121609.6	1	UniProt (Q61115) Pfam (PF12349)	Mus musculus (Mouse)	PTC,PTC1,PTC,PTC1
2	B (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	C, D, E (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
4	F (A)	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)

Sequence modifications

A: 2 - 709 (UniProt: Q61115)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ser 1	-	expression tag

A: 710 - 1175 (UniProt: Q61115)

PDB	External Database	Details
Ala 1084	Val 1084	engineered mutation
Thr 1176	-	expression tag
Leu 1177	-	expression tag
Glu 1178	-	expression tag
Val 1179	-	expression tag
Leu 1180	-	expression tag
Phe 1181	-	expression tag
Gln 1182	-	expression tag
Gly 1183	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	121609.6
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1050.3
All*	Total formula weight	123084.2

*Water molecules are not included.