7V69

Cryo-EM structure of a class A GPCR-G protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	356	40559.2	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	339	37285.7	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (S)	scFv16	polymer	259	27784.9	1		Homo sapiens
5	E (R)	Muscarinic acetylcholine receptor M4	polymer	346	39055.0	1	UniProt (P08173) Pfam (PF00001)	Homo sapiens (human)

Sequence modifications

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag

R: 1 - 479 (UniProt: P08173)

PDB	External Database	Details
-	Lys 240	deletion
-	Thr 241	deletion
-	Leu 242	deletion
-	Ala 243	deletion
-	Phe 244	deletion
-	Leu 245	deletion
-	Lys 246	deletion
-	Ser 247	deletion
-	Pro 248	deletion
-	Leu 249	deletion
-	Met 250	deletion
-	Lys 251	deletion
-	Gln 252	deletion
-	Ser 253	deletion
-	Val 254	deletion
-	Lys 255	deletion
-	Lys 256	deletion
-	Pro 257	deletion
-	Pro 258	deletion
-	Pro 259	deletion
-	Gly 260	deletion
-	Glu 261	deletion
-	Ala 262	deletion
-	Ala 263	deletion
-	Arg 264	deletion
-	Glu 265	deletion
-	Glu 266	deletion
-	Leu 267	deletion
-	Arg 268	deletion
-	Asn 269	deletion
-	Gly 270	deletion
-	Lys 271	deletion
-	Leu 272	deletion
-	Glu 273	deletion
-	Glu 274	deletion
-	Ala 275	deletion
-	Pro 276	deletion
-	Pro 277	deletion
-	Pro 278	deletion
-	Ala 279	deletion
-	Leu 280	deletion
-	Pro 281	deletion
-	Pro 282	deletion
-	Pro 283	deletion
-	Pro 284	deletion
-	Arg 285	deletion
-	Pro 286	deletion
-	Val 287	deletion
-	Ala 288	deletion
-	Asp 289	deletion
-	Lys 290	deletion
-	Asp 291	deletion
-	Thr 292	deletion
-	Ser 293	deletion
-	Asn 294	deletion
-	Glu 295	deletion
-	Ser 296	deletion
-	Ser 297	deletion
-	Ser 298	deletion
-	Gly 299	deletion
-	Ser 300	deletion
-	Ala 301	deletion
-	Thr 302	deletion
-	Gln 303	deletion
-	Asn 304	deletion
-	Thr 305	deletion
-	Lys 306	deletion
-	Glu 307	deletion
-	Arg 308	deletion
-	Pro 309	deletion
-	Ala 310	deletion
-	Thr 311	deletion
-	Glu 312	deletion
-	Leu 313	deletion
-	Ser 314	deletion
-	Thr 315	deletion
-	Thr 316	deletion
-	Glu 317	deletion
-	Ala 318	deletion
-	Thr 319	deletion
-	Thr 320	deletion
-	Pro 321	deletion
-	Ala 322	deletion
-	Met 323	deletion
-	Pro 324	deletion
-	Ala 325	deletion
-	Pro 326	deletion
-	Pro 327	deletion
-	Leu 328	deletion
-	Gln 329	deletion
-	Pro 330	deletion
-	Arg 331	deletion
-	Ala 332	deletion
-	Leu 333	deletion
-	Asn 334	deletion
-	Pro 335	deletion
-	Ala 336	deletion
-	Ser 337	deletion
-	Arg 338	deletion
-	Trp 339	deletion
-	Ser 340	deletion
-	Lys 341	deletion
-	Ile 342	deletion
-	Gln 343	deletion
-	Ile 344	deletion
-	Val 345	deletion
-	Thr 346	deletion
-	Lys 347	deletion
-	Gln 348	deletion
-	Thr 349	deletion
-	Gly 350	deletion
-	Asn 351	deletion
-	Glu 352	deletion
-	Cys 353	deletion
-	Val 354	deletion
-	Thr 355	deletion
-	Ala 356	deletion
-	Ile 357	deletion
-	Glu 358	deletion
-	Ile 359	deletion
-	Val 360	deletion
-	Pro 361	deletion
-	Ala 362	deletion
-	Thr 363	deletion
-	Pro 364	deletion
-	Ala 365	deletion
-	Gly 366	deletion
-	Met 367	deletion
-	Arg 368	deletion
-	Pro 369	deletion
-	Ala 370	deletion
-	Ala 371	deletion
-	Asn 372	deletion

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	152546.0
All*	Total formula weight	152546.0

*Water molecules are not included.