7V5S

Crystal structure of human bleomycin hydrolase C73A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Bleomycin hydrolase	polymer	475	54767.4	1	UniProt (Q13867) Pfam (PF03051)	Homo sapiens (Human)	BH,BLM hydrolase,BMH
2	B (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
3	C (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
4	D (A)	water	water		18.0	19	Chemie (HOH)

Sequence modifications

A: 1 - 455 (UniProt: Q13867)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 73	Cys 73	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	54767.4
Non-Polymers*	Number of molecules	2
	Total formula weight	344.4
All*	Total formula weight	55111.8

*Water molecules are not included.