7V35
Cryo-EM structure of the GIPR/GLP-1R/GCGR triagonist peptide 20-bound human GCGR-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 394 | 45683.4 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (Human) | Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P54311) Pfam (PF25391) | Rattus norvegicus (Rat) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G gamma-I |
| 4 | D (N) | Nanobody-35 | polymer | 140 | 15343.0 | 1 | synthetic construct | ||
| 5 | E (P) | Peptide 20 | polymer | 39 | 4180.5 | 1 | synthetic construct | ||
| 6 | F (R) | Glucagon receptor | polymer | 406 | 46741.4 | 1 | UniProt (P47871) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (Human) | GL-R |
| 7 | G (P) | N-hexadecanoyl-L-glutamic acid | non-polymer | 385.5 | 1 | Chemie (D6M) |
Sequence modifications
A: 1 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| Asn 54 | Ser 54 | engineered mutation |
| Ala 226 | Gly 226 | engineered mutation |
| Ala 268 | Glu 268 | engineered mutation |
| Lys 271 | Asn 271 | engineered mutation |
| Asp 274 | Lys 274 | engineered mutation |
| Lys 280 | Arg 280 | engineered mutation |
| Asp 284 | Thr 284 | engineered mutation |
| Thr 285 | Ile 285 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 157725.0 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 385.5 | |
| All* | Total formula weight | 158110.5 |
