7V24

Local CryoEM structure of del68-76/del679-688 prefusion-stabilized spike in complex with the Fab of N12-9

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	the heavy chain of N12-9 Fab	polymer	125	13487.9	1		Homo sapiens
2	B (L)	the lignt chain of N12-9 Fab	polymer	106	11209.3	1		Homo sapiens
3	C (A)	Spike glycoprotein	polymer	1269	140701.6	1	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	S glycoprotein,E2,Peplomer protein

Sequence modifications

A: 1 - 1198 (UniProt: P0DTC2)

PDB	External Database	Details
-	Ile 68	deletion
-	His 69	deletion
-	Val 70	deletion
-	Ser 71	deletion
-	Gly 72	deletion
-	Thr 73	deletion
-	Asn 74	deletion
-	Gly 75	deletion
-	Thr 76	deletion
-	Asn 679	deletion
-	Ser 680	deletion
-	Pro 681	deletion
-	Arg 682	deletion
-	Arg 683	deletion
-	Ala 684	deletion
-	Arg 685	deletion
-	Ser 686	deletion
-	Val 687	deletion
-	Ala 688	deletion
Pro 807	Phe 817	engineered mutation
Pro 882	Ala 892	engineered mutation
Pro 889	Ala 899	engineered mutation
Pro 932	Ala 942	engineered mutation
Pro 976	Lys 986	engineered mutation
Pro 977	Val 987	engineered mutation
Gly 1199	-	expression tag
Ser 1200	-	expression tag
Gly 1201	-	expression tag
Tyr 1202	-	expression tag
Ile 1203	-	expression tag
Pro 1204	-	expression tag
Glu 1205	-	expression tag
Ala 1206	-	expression tag
Pro 1207	-	expression tag
Arg 1208	-	expression tag
Asp 1209	-	expression tag
Gly 1210	-	expression tag
Gln 1211	-	expression tag
Ala 1212	-	expression tag
Tyr 1213	-	expression tag
Val 1214	-	expression tag
Arg 1215	-	expression tag
Lys 1216	-	expression tag
Asp 1217	-	expression tag
Gly 1218	-	expression tag
Glu 1219	-	expression tag
Trp 1220	-	expression tag
Val 1221	-	expression tag
Leu 1222	-	expression tag
Leu 1223	-	expression tag
Ser 1224	-	expression tag
Thr 1225	-	expression tag
Phe 1226	-	expression tag
Leu 1227	-	expression tag
Gly 1228	-	expression tag
Arg 1229	-	expression tag
Ser 1230	-	expression tag
Leu 1231	-	expression tag
Glu 1232	-	expression tag
Val 1233	-	expression tag
Leu 1234	-	expression tag
Phe 1235	-	expression tag
Gln 1236	-	expression tag
Gly 1237	-	expression tag
Pro 1238	-	expression tag
Gly 1239	-	expression tag
His 1240	-	expression tag
His 1241	-	expression tag
His 1242	-	expression tag
His 1243	-	expression tag
His 1244	-	expression tag
His 1245	-	expression tag
His 1246	-	expression tag
His 1247	-	expression tag
Ser 1248	-	expression tag
Ala 1249	-	expression tag
Trp 1250	-	expression tag
Ser 1251	-	expression tag
His 1252	-	expression tag
Pro 1253	-	expression tag
Gln 1254	-	expression tag
Phe 1255	-	expression tag
Glu 1256	-	expression tag
Lys 1257	-	expression tag
Gly 1258	-	expression tag
Gly 1259	-	expression tag
Gly 1260	-	expression tag
Ser 1261	-	expression tag
Gly 1262	-	expression tag
Gly 1263	-	expression tag
Gly 1264	-	expression tag
Gly 1265	-	expression tag
Ser 1266	-	expression tag
Gly 1267	-	expression tag
Gly 1268	-	expression tag
Ser 1269	-	expression tag
Ala 1270	-	expression tag
Trp 1271	-	expression tag
Ser 1272	-	expression tag
His 1273	-	expression tag
Pro 1274	-	expression tag
Gln 1275	-	expression tag
Phe 1276	-	expression tag
Glu 1277	-	expression tag
Lys 1278	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	165398.9
All*	Total formula weight	165398.9

*Water molecules are not included.