7UV7
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and scaffold duplex (21mer) containing the cognate REPE54 sequence and an insert duplex (10mer) with guest TAMRA-labelled thymine and T-A rich sequence.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA (5'-D(*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*A)-3') | polymer | 21 | 6367.1 | 1 | Escherichia coli | ||
| 2 | B (B) | DNA (5'-D(*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*G)-3') | polymer | 21 | 6478.2 | 1 | Escherichia coli | ||
| 3 | C (C) | Replication initiation protein | polymer | 263 | 30749.1 | 1 | UniProt (P03856) Pfam (PF01051) Pfam (PF21205) | Escherichia coli K-12 | Protein E,Protein rep,Protein F4 |
| 4 | D (E) | DNA (5'-D(*GP*AP*CP*GP*GP*TP*AP*AP*TP*T)-3') | polymer | 10 | 3084.0 | 1 | Escherichia coli | ||
| 5 | E (F) | DNA (5'-D(*GP*T(TAMRA)P*AP*AP*TP*TP*AP*CP*CP*G)-3') | polymer | 10 | 3044.0 | 1 | Escherichia coli | ||
| 6 | F (C) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 7 | G, H, I (A, B, C) | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
C: 1 - 251 (UniProt: P03856)
| PDB | External Database | Details |
|---|---|---|
| Met -11 | - | initiating methionine |
| Arg -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Ser -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Pro 118 | Arg 118 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 49722.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 49746.7 |
