7UV6

Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and scaffold duplex (21mer) containing the cognate REPE54 sequence and an insert duplex (10mer) with guest TAMRA-labelled thymine and G-C rich sequence.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA (5'-D(*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*A)-3')	polymer	21	6367.1	1		Escherichia coli
2	B (B)	DNA (5'-D(*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*G)-3')	polymer	21	6478.2	1		Escherichia coli
3	C (C)	Replication initiation protein	polymer	263	30749.1	1	UniProt (P03856) Pfam (PF01051) Pfam (PF21205)	Escherichia coli K-12	Protein E,Protein rep,Protein F4
4	D (E)	DNA (5'-D(*GP*AP*CP*GP*GP*CP*CP*CP*GP*G)-3')	polymer	10	3071.0	1		Escherichia coli
5	E (F)	DNA (5'-D(*GP*T(TAMRA)P*CP*CP*GP*GP*GP*CP*CP*G)-3')	polymer	10	3062.0	1		Escherichia coli
6	F, G, H, I, J (A, B, C)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
7	K, L, M, N (A, B, C, E)	water	water		18.0	33	Chemie (HOH)

Sequence modifications

C: 1 - 251 (UniProt: P03856)

PDB	External Database	Details
Met -11	-	initiating methionine
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Pro 118	Arg 118	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	49727.4
Non-Polymers*	Number of molecules	5
	Total formula weight	121.5
All*	Total formula weight	49848.9

*Water molecules are not included.