7UR4

Cryo-EM Structure of the Neutralizing Antibody MPV467 in Complex with Prefusion Human Metapneumovirus F Glycoprotein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Fusion glycoprotein F0	polymer	551	60489.9	3	UniProt (H6X1Z1) Pfam (PF00523) UniProt (by SIFTS) (Q6WB98)	Human metapneumovirus
2	D, F, G (F, H, I)	MPV467 Fab Heavy chain	polymer	223	24023.8	3		Homo sapiens (Human)
3	E, H, I (G, J, L)	MPV467 Fab Light chain	polymer	211	22621.9	3		Homo sapiens (Human)
4	J, K, L (D, E, K)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)
5	M, N, O, P, Q... (A, B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 490 (UniProt: H6X1Z1)

PDB	External Database	Details
Cys 84	Val 84	engineered mutation
Arg 100	Gln 100	engineered mutation
Arg 101	Ser 101	engineered mutation
Cys 110	Leu 110	engineered mutation
Cys 127	Thr 127	engineered mutation
Cys 140	Ala 140	engineered mutation
Cys 147	Ala 147	engineered mutation
Cys 153	Asn 153	engineered mutation
Pro 185	Ala 185	engineered mutation
Lys 219	Leu 219	engineered mutation
Ile 231	Val 231	engineered mutation
Cys 249	Ala 249	engineered mutation
Cys 322	Asn 322	engineered mutation
Cys 365	Thr 365	engineered mutation
Gln 453	Glu 453	engineered mutation
Cys 463	Val 463	engineered mutation
Gly 491	-	expression tag
Gly 492	-	expression tag
Gly 493	-	expression tag
Ser 494	-	expression tag
Gly 495	-	expression tag
Tyr 496	-	expression tag
Ile 497	-	expression tag
Pro 498	-	expression tag
Glu 499	-	expression tag
Ala 500	-	expression tag
Pro 501	-	expression tag
Arg 502	-	expression tag
Asp 503	-	expression tag
Gly 504	-	expression tag
Gln 505	-	expression tag
Ala 506	-	expression tag
Tyr 507	-	expression tag
Val 508	-	expression tag
Arg 509	-	expression tag
Lys 510	-	expression tag
Asp 511	-	expression tag
Gly 512	-	expression tag
Glu 513	-	expression tag
Trp 514	-	expression tag
Val 515	-	expression tag
Leu 516	-	expression tag
Leu 517	-	expression tag
Ser 518	-	expression tag
Thr 519	-	expression tag
Phe 520	-	expression tag
Leu 521	-	expression tag
Gly 522	-	expression tag
Arg 523	-	expression tag
Ser 524	-	expression tag
Leu 525	-	expression tag
Glu 526	-	expression tag
Val 527	-	expression tag
Leu 528	-	expression tag
Phe 529	-	expression tag
Gln 530	-	expression tag
Gly 531	-	expression tag
Pro 532	-	expression tag
Gly 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
Ser 542	-	expression tag
Ala 543	-	expression tag
Trp 544	-	expression tag
Ser 545	-	expression tag
His 546	-	expression tag
Pro 547	-	expression tag
Gln 548	-	expression tag
Phe 549	-	expression tag
Glu 550	-	expression tag
Lys 551	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	321406.9
Branched	Number of molecules	3
	Total formula weight	1759.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	324493.8

*Water molecules are not included.