7UR0
Isoreticular, interpenetrating co-crystal of Replication Initiator Protein REPE54 and scaffold-insert duplexes (21mer and 10mer) containing the cognate REPE54 sequence and an additional T-A rich sequence, respectively.
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA (5'-D(*AP*AP*CP*CP*TP*GP*TP*GP*AP*CP*AP*AP*AP*TP*TP*GP*CP*CP*CP*TP*CP*A)-3') | polymer | 22 | 6680.3 | 1 | Escherichia coli | ||
2 | B | DNA (5'-D(A*TP*CP*TP*GP*AP*GP*GP*GP*CP*AP*AP*TP*TP*TP*GP*TP*CP*AP*CP*AP*G)-3') | polymer | 22 | 6791.4 | 1 | Escherichia coli | ||
3 | C | Replication initiation protein | polymer | 263 | 30749.1 | 1 | UniProt (P03856) Pfam (PF01051) Pfam (PF21205) In PDB | Escherichia coli K-12 | Protein E,Protein rep,Protein F4 |
4 | E | DNA (5'-D(A*GP*AP*CP*GP*GP*TP*AP*AP*TP*T)-3') | polymer | 11 | 3397.2 | 1 | Escherichia coli | ||
5 | F | DNA (5'-D(A*GP*TP*AP*AP*TP*TP*AP*CP*CP*G)-3') | polymer | 11 | 3357.2 | 1 | Escherichia coli | ||
6 | C | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
7 | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
C: 1 - 251 (UniProt: P03856)
PDB | External Database | Details |
---|---|---|
Met -11 | - | initiating methionine |
Arg -10 | - | expression tag |
Gly -9 | - | expression tag |
Ser -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
Gly -1 | - | expression tag |
Ser 0 | - | expression tag |
Pro 118 | Arg 118 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 50975.3 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 48.6 | |
All* | Total formula weight | 51023.9 |