7UPY

An antibody from single human VH-rearranging mouse neutralizes all SARS-CoV-2 variants through BA.5 by inhibiting membrane fusion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Spike glycoprotein	polymer	1310	144858.0	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Severe acute respiratory syndrome coronavirus	S glycoprotein,E2,Peplomer protein
2	H, D, F	SP1-77 Fab heavy chain	polymer	451	49665.7	3		Homo sapiens (Human)
3	L, E, G	SP1-77 Fab light chain	polymer	213	23294.6	3		Homo sapiens (Human)
4	f, h, i, I, j...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	16	In PDB GlyTouCan (G42666HT)
5	b, c, e, g, J...	alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	12	In PDB GlyTouCan (G62182OO)
6	a, d, m, P, p...	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	6	In PDB GlyTouCan (G21290RB)
7	B, C, A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	23	Chemie (NAG)

Sequence modifications

A, B, C: 1 - 1273 (UniProt: P0DTC2)

PDB	External Database	Details
Gly 614	Asp 614	engineered mutation
Leu 1274	-	expression tag
Glu 1275	-	expression tag
Ser 1276	-	expression tag
Gly 1277	-	expression tag
Gly 1278	-	expression tag
Gly 1279	-	expression tag
Ser 1280	-	expression tag
Ala 1281	-	expression tag
Trp 1282	-	expression tag
Ser 1283	-	expression tag
His 1284	-	expression tag
Pro 1285	-	expression tag
Gln 1286	-	expression tag
Phe 1287	-	expression tag
Glu 1288	-	expression tag
Lys 1289	-	expression tag
Gly 1290	-	expression tag
Gly 1291	-	expression tag
Gly 1292	-	expression tag
Ser 1293	-	expression tag
Gly 1294	-	expression tag
Gly 1295	-	expression tag
Gly 1296	-	expression tag
Ser 1297	-	expression tag
Gly 1298	-	expression tag
Gly 1299	-	expression tag
Ser 1300	-	expression tag
Ser 1301	-	expression tag
Ala 1302	-	expression tag
Trp 1303	-	expression tag
Ser 1304	-	expression tag
His 1305	-	expression tag
Pro 1306	-	expression tag
Gln 1307	-	expression tag
Phe 1308	-	expression tag
Glu 1309	-	expression tag
Lys 1310	-	expression tag

H, D, F: 1 - 90 (PDB: 7UPY)
H, D, F: 91 - 102 (PDB: 7UPY)
H, D, F: 103 - 440 (PDB: 7UPY)
L, E, G: 1 - 97 (PDB: 7UPY)
L, E, G: 98 - 108 (PDB: 7UPY)
L, E, G: 109 - 214 (PDB: 7UPY)

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	653455.1
Branched	Number of molecules	34
	Total formula weight	17252.2
Non-Polymers*	Number of molecules	23
	Total formula weight	5087.8
All*	Total formula weight	675795.0

*Water molecules are not included.