7UOJ
The CryoEM structure of N49-P9.6-FR3 and PGT121 Fabs in complex with BG505 SOSIP.664
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, G, M (G, A, I) | Envelope glycoprotein gp120 | polymer | 481 | 54064.3 | 3 | UniProt (Q2N0S6) Pfam (PF00516) | Human immunodeficiency virus 1 | |
| 2 | B, H, N (B, C, J) | Envelope glycoprotein gp41 | polymer | 153 | 17146.5 | 3 | UniProt (Q2N0S6) Pfam (PF00517) | Human immunodeficiency virus 1 | Env polyprotein |
| 3 | C, I, O (H, D, K) | N49-P9.6-FR3 Fab heavy chain | polymer | 230 | 25024.1 | 3 | Homo sapiens | ||
| 4 | D, J, P (L, E, M) | N49-P9.6-FR3 Fab light chain | polymer | 203 | 21733.3 | 3 | Homo sapiens | ||
| 5 | E, K, Q (l, e, m) | PGT121 Fab light chain | polymer | 213 | 22869.3 | 3 | Homo sapiens | ||
| 6 | F, L, R (h, d, k) | PGT121 Fab heavy chain | polymer | 235 | 25169.2 | 3 | Homo sapiens | ||
| 7 | BA, CA, DA, EA, GA... (V, W, X, Y, a...) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 21 | In PDB GlyTouCan (G42666HT) | |||
| 8 | FA, QA, U (Z, q, O) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 3 | In PDB GlyTouCan (G81315DD) | |||
| 9 | IA, TA, X (c, t, R) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 3 | In PDB GlyTouCan (G15407YE) | |||
| 10 | JA, UA, Y (f, u, S) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 3 | In PDB GlyTouCan (G22768VO) | |||
| 11 | AA, LA, WA (U, i, w) | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1559.4 | 3 | In PDB GlyTouCan (G68668TB) | |||
| 12 | AB, BB, CB, DB, EB... (G, A, I) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 15 | Chemie (NAG) |
Sequence modifications
G, A, I: 31 - 508 (UniProt: Q2N0S6)
B, C, J: 512 - 664 (UniProt: Q2N0S6)
| PDB | External Database | Details |
|---|---|---|
| Asn 332 | Thr 330 | engineered mutation |
| Cys 501 | Ala 498 | engineered mutation |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Arg 511 | - | expression tag |
| Arg 512 | - | expression tag |
| Arg 513 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 559 | Ile 556 | engineered mutation |
| Cys 605 | Thr 602 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 18 |
| Total formula weight | 498020.2 | |
| Branched | Number of molecules | 33 |
| Total formula weight | 20328.7 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 3318.1 | |
| All* | Total formula weight | 521667.1 |
