7UOB

SARS-CoV-2 replication-transcription complex bound to GTP, in a pre-catalytic state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA-directed RNA polymerase	polymer	932	106781.0	1	UniProt (P0DTD1) Pfam (PF06478) Pfam (PF00680)	Severe acute respiratory syndrome coronavirus 2	Pol, RdRp, Non-structural protein 12, nsp12
2	B, D (B, D)	Non-structural protein 8	polymer	198	21903.0	2	UniProt (P0DTD1) Pfam (PF08717)	Severe acute respiratory syndrome coronavirus 2	nsp8
3	C (C)	Non-structural protein 7	polymer	92	10250.0	1	UniProt (P0DTD1) Pfam (PF08716)	Severe acute respiratory syndrome coronavirus 2	nsp7
4	E (P)	Product RNA (35-MER)	polymer	35	11155.7	1		synthetic construct
5	F (T)	Template RNA (55-MER)	polymer	55	17557.3	1		synthetic construct
6	G, H (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	I, J, K (A)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
8	L, M (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	2	Chemie (GTP)
9	N (P)	3'-DEOXYURIDINE-5'-MONOPHOSPHATE	non-polymer		308.2	1	Chemie (L2B)
10	O, P, Q, R, S... (A, B, C, D, P...)	water	water		18.0	288	Chemie (HOH)

Sequence modifications

C: 1 - 83 (UniProt: P0DTD1)

PDB	External Database	Details
Val -8	-	expression tag
Ala -7	-	expression tag
Cys -6	-	expression tag
Thr -5	-	expression tag
Lys -4	-	expression tag
Glu -3	-	expression tag
Val -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	189550.1
Non-Polymers*	Number of molecules	8
	Total formula weight	1558.3
All*	Total formula weight	191108.4

*Water molecules are not included.