7UM0

Structure of the phage AR9 non-virion RNA polymerase holoenzyme in complex with two DNA oligonucleotides containing the AR9 P077 promoter as determined by cryo-EM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed RNA polymerase subunit	polymer	464	54917.4	1	UniProt (A0A172JIC8) In PDB	Bacillus phage AR9
2	d	DNA-directed RNA polymerase beta' subunit	polymer	448	51947.8	1	UniProt (A0A172JIH0) In PDB	Bacillus phage AR9
3	c	DNA-directed RNA polymerase beta subunit	polymer	496	58112.3	1	UniProt (A0A172JI16) In PDB	Bacillus phage AR9
4	D	DNA-directed RNA polymerase	polymer	631	73101.0	1	UniProt (A0A172JI62) Pfam (PF04998) In PDB	Bacillus phage AR9
5	C	DNA-directed RNA polymerase	polymer	665	76978.4	1	UniProt (A0A172JHZ2) Pfam (PF00562) In PDB	Bacillus phage AR9
6	B	DNA (5'-D(P*GP*UP*U)-3')	polymer	3	864.6	1		synthetic construct
7	D	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

d: 1 - 426 (UniProt: A0A172JIH0)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	315921.5
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	315986.9

*Water molecules are not included.