7ULC
Crystal structure of queuine salvage enzyme DUF2419 mutant D231N, in complex with queuosine-5'-monophosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Queuosine salvage protein DUF2419 | polymer | 331 | 37187.7 | 2 | UniProt (D1C7A6) Pfam (PF10343) | Sphaerobacter thermophilus DSM 20745 | |
| 2 | C, E (A, B) | 2-amino-5-({[(1S,4S,5R)-4,5-dihydroxycyclopent-2-en-1-yl]amino}methyl)-7-(5-O-phosphono-beta-D-ribofuranosyl)-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one | non-polymer | 489.4 | 2 | Chemie (56B) | |||
| 3 | D (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 4 | F, G (A, B) | water | water | 18.0 | 265 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 323 (UniProt: D1C7A6)
| PDB | External Database | Details |
|---|---|---|
| Asn 231 | Asp 231 | engineered mutation |
| Leu 324 | - | expression tag |
| Glu 325 | - | expression tag |
| His 326 | - | expression tag |
| His 327 | - | expression tag |
| His 328 | - | expression tag |
| His 329 | - | expression tag |
| His 330 | - | expression tag |
| His 331 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 74375.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 1084.9 | |
| All* | Total formula weight | 75460.3 |
