7ULC

Crystal structure of queuine salvage enzyme DUF2419 mutant D231N, in complex with queuosine-5'-monophosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Queuosine salvage protein DUF2419	polymer	331	37187.7	2	UniProt (D1C7A6) Pfam (PF10343) In PDB	Sphaerobacter thermophilus DSM 20745
2	A, B	2-amino-5-({[(1S,4S,5R)-4,5-dihydroxycyclopent-2-en-1-yl]amino}methyl)-7-(5-O-phosphono-beta-D-ribofuranosyl)-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one	non-polymer		489.4	2	Chemie (56B)
3	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
4		water	water		18.0	265	Chemie (HOH)

Sequence modifications

A, B: 1 - 323 (UniProt: D1C7A6)

PDB	External Database	Details
Asn 231	Asp 231	engineered mutation
Leu 324	-	expression tag
Glu 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74375.4
Non-Polymers*	Number of molecules	3
	Total formula weight	1084.9
All*	Total formula weight	75460.3

*Water molecules are not included.