7UDC

cryo-EM structures of a synaptobrevin-Munc18-1-syntaxin-1 complex class1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Syntaxin-binding protein 1	polymer	607	68714.9	1	UniProt (P61765) Pfam (PF00995) In PDB	Rattus norvegicus (Norway rat)	N-Sec1,Protein unc-18 homolog 1,Unc18-1,Protein unc-18 homolog A,Unc-18A,p67,rbSec1
2	B	Syntaxin-1A	polymer	254	29221.5	1	UniProt (P32851) Pfam (PF00804) Pfam (PF05739) In PDB	Rattus norvegicus (Norway rat)	Neuron-specific antigen HPC-1,Synaptotagmin-associated 35 kDa protein,P35A
3	C	Synaptobrevin-2	polymer	59	6645.4	1	UniProt (P63027) Pfam (PF00957) In PDB	Rattus norvegicus (Norway rat)	VAMP-2,Vesicle-associated membrane protein 2

Sequence modifications

A: 1 - 594 (UniProt: P61765)

PDB	External Database	Details
Gly -12	-	expression tag
Ser -11	-	expression tag
Pro -10	-	expression tag
Gly -9	-	expression tag
Ile -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ile -1	-	expression tag
His 0	-	expression tag
Lys 326	Asp 326	conflict

B: 2 - 253 (UniProt: P32851)

PDB	External Database	Details
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 145	Cys 145	engineered mutation
Ala 165	Leu 165	engineered mutation
Ala 166	Glu 166	engineered mutation
Cys 205	Leu 205	engineered mutation

C: 29 - 83 (UniProt: P63027)

PDB	External Database	Details
Gly 25	-	expression tag
Ser 26	-	expression tag
Met 27	-	expression tag
Trp 28	-	expression tag
Cys 36	Gln 36	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	104581.8
All*	Total formula weight	104581.8

*Water molecules are not included.