7UBN

Transcription antitermination complex: NusA-containing "engaged" Qlambda-loading complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	1	DNA (53-MER)	polymer	61	18933.2	1		Escherichia phage Lambda
2	2	DNA (52-MER)	polymer	61	18601.0	1		Escherichia phage Lambda
3	A, B	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	2	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
4	C	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	1	UniProt (P0A8V4) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
5	D	DNA-directed RNA polymerase subunit beta'	polymer	1430	158314.9	1	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
6	E	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	1	UniProt (P0A802) Pfam (PF01192) UniProt (by SIFTS) (P0A800) In PDB	Escherichia coli	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
7	F	RNA polymerase sigma factor RpoD	polymer	627	71912.9	1	UniProt (Q0P6L9) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) UniProt (by SIFTS) (P00579) In PDB	Escherichia coli	Sigma-70
8	N	Transcription termination/antitermination protein NusA	polymer	515	57104.0	1	UniProt (C3SSN7) Pfam (PF08529) Pfam (PF00575) Pfam (PF13184) Pfam (PF14520) UniProt (by SIFTS) (P0AFF6) In PDB	Escherichia coli
9	Q	Antitermination protein	polymer	207	22506.0	1	UniProt (A0A0L6XR38) Pfam (PF03589) UniProt (by SIFTS) (P03047) In PDB	Escherichia coli	Late gene regulator
10	R	RNA (5'-R(*UP*GP*GP*GP*AP*GP*AP*GP*GP*UP*A)-3')	polymer	11	3626.2	1		Escherichia coli
11	D, Q	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
12	D	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

D: 1 - 1407 (UniProt: P0A8T7)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Arg 1410	-	expression tag
Arg 1411	-	expression tag
Ala 1412	-	expression tag
Ser 1413	-	expression tag
Glu 1414	-	expression tag
Asn 1415	-	expression tag
Leu 1416	-	expression tag
Tyr 1417	-	expression tag
Phe 1418	-	expression tag
Gln 1419	-	expression tag
Gly 1420	-	expression tag
His 1421	-	expression tag
His 1422	-	expression tag
His 1423	-	expression tag
His 1424	-	expression tag
His 1425	-	expression tag
His 1426	-	expression tag
His 1427	-	expression tag
His 1428	-	expression tag
His 1429	-	expression tag
His 1430	-	expression tag

F: 1 - 613 (UniProt: Q0P6L9)

PDB	External Database	Details
Met -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag

N: 1 - 495 (UniProt: C3SSN7)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	585186.0
Non-Polymers*	Number of molecules	4
	Total formula weight	220.5
All*	Total formula weight	585406.5

*Water molecules are not included.