7U5A

Crystal structure of queuine salvage enzyme DUF2419 mutant K199C, complexed with queuosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Queuine salvage enzyme DUF2419	polymer	331	37162.6	2	UniProt (D1C7A6) Pfam (PF10343)	Sphaerobacter thermophilus DSM 20745
2	C, E (A, B)	2-amino-5-({[(1S,4S,5R)-4,5-dihydroxycyclopent-2-en-1-yl]amino}methyl)-7-beta-D-ribofuranosyl-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one	non-polymer		409.4	2	Chemie (QEO)
3	D (A)	MALONATE ION	non-polymer		102.0	1	Chemie (MLI)
4	F, G (A, B)	water	water		18.0	191	Chemie (HOH)

Sequence modifications

A, B: 1 - 323 (UniProt: D1C7A6)

PDB	External Database	Details
Cys 199	Lys 199	engineered mutation
Leu 324	-	expression tag
Glu 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74325.3
Non-Polymers*	Number of molecules	3
	Total formula weight	920.8
All*	Total formula weight	75246.1

*Water molecules are not included.