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7U5A

Crystal structure of queuine salvage enzyme DUF2419 mutant K199C, complexed with queuosine

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, BQueuine salvage enzyme DUF2419polymer33137162.62UniProt (D1C7A6)
Pfam (PF10343)
In PDB
Sphaerobacter thermophilus DSM 20745
2A, B2-amino-5-({[(1S,4S,5R)-4,5-dihydroxycyclopent-2-en-1-yl]amino}methyl)-7-beta-D-ribofuranosyl-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-onenon-polymer409.42Chemie (QEO)
3AMALONATE IONnon-polymer102.01Chemie (MLI)
4waterwater18.0191Chemie (HOH)
Sequence modifications
A, B: 1 - 323 (UniProt: D1C7A6)
PDBExternal DatabaseDetails
Cys 199Lys 199engineered mutation
Leu 324-expression tag
Glu 325-expression tag
His 326-expression tag
His 327-expression tag
His 328-expression tag
His 329-expression tag
His 330-expression tag
His 331-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains2
Total formula weight74325.3
Non-Polymers*Number of molecules3
Total formula weight920.8
All*Total formula weight75246.1
*Water molecules are not included.

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PDB entries from 2024-07-24

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