7U47

CryoEM structure of CENP-N promoted nucleosome stacks with CENP-A and palindromic alpha satellite DNA sequence

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, L, P	Histone H3-like centromeric protein A	polymer	140	16023.6	4	UniProt (P49450) Pfam (PF00125) In PDB	Homo sapiens (human)	Centromere autoantigen A,Centromere protein A,CENP-A
2	B, F, M, Q	Histone H4	polymer	103	11394.4	4	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (human)
3	C, G, N, R	Histone H2A	polymer	130	14135.5	4	UniProt (Q93077) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (human)	Histone H2A/l
4	D, H, O, S	Histone H2B type 1-C/E/F/G/I	polymer	126	13937.2	4	UniProt (P62807) Pfam (PF00125) In PDB	Homo sapiens (human)	Histone H2B.1 A,Histone H2B.a,H2B/a,Histone H2B.g,H2B/g,Histone H2B.h,H2B/h,Histone H2B.k,H2B/k,Histone H2B.l,H2B/l
5	I, T	DNA (147-MER)	polymer	147	45368.1	2		Escherichia coli
6	J, U	DNA (147-MER)	polymer	147	45359.0	2		Escherichia coli
7	K, V	Centromere protein N	polymer	295	34870.7	2	UniProt (Q96H22) Pfam (PF05238) In PDB	Homo sapiens (human)	CENP-N,Interphase centromere complex protein 32

Sequence modifications

K, V: 1 - 289 (UniProt: Q96H22)

PDB	External Database	Details
Asp 84	Glu 84	variant
His 290	-	expression tag
His 291	-	expression tag
His 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	22
	Total formula weight	473158.7
All*	Total formula weight	473158.7

*Water molecules are not included.