7U2P

Structure of TcdA GTD in complex with RhoA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glucosyltransferase TcdA	polymer	543	62919.0	1	UniProt (P16154) Pfam (PF12918) Pfam (PF12919) In PDB	Clostridioides difficile
2	B	Transforming protein RhoA	polymer	186	20891.9	1	UniProt (Q9QUI0) Pfam (PF00071) UniProt (by SIFTS) (P61586) In PDB	Homo sapiens (human)
3	A	URIDINE-5'-DIPHOSPHATE-GLUCOSE	non-polymer		566.3	1	Chemie (UPG)
4	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
5	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
6	B	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
7	B	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8		water	water		18.0	52	Chemie (HOH)

Sequence modifications

A: 1 - 542 (UniProt: P16154)

PDB	External Database	Details
Ser 0	-	expression tag
Ala 190	Lys 190	engineered mutation

B: 1 - 181 (UniProt: Q9QUI0)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asn 37	Thr 37	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	83810.9
Non-Polymers*	Number of molecules	5
	Total formula weight	1184.8
All*	Total formula weight	84995.7

*Water molecules are not included.