7U1O

Crystal structure of queuine salvage enzyme DUF2419 complexed with queuosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Queuine salvage enzyme DUF2419	polymer	331	37188.7	2	UniProt (D1C7A6) Pfam (PF10343) In PDB	Sphaerobacter thermophilus DSM 20745
2	A, B	2-amino-5-({[(1S,4S,5R)-4,5-dihydroxycyclopent-2-en-1-yl]amino}methyl)-7-beta-D-ribofuranosyl-3,7-dihydro-4H-pyrrolo[2,3-d]pyrimidin-4-one	non-polymer		409.4	2	Chemie (QEO)
3	A, B	MALONATE ION	non-polymer		102.0	2	Chemie (MLI)
4	B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5		water	water		18.0	267	Chemie (HOH)

Sequence modifications

A, B: 1 - 323 (UniProt: D1C7A6)

PDB	External Database	Details
Leu 324	-	expression tag
Glu 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74377.4
Non-Polymers*	Number of molecules	5
	Total formula weight	1129.0
All*	Total formula weight	75506.4

*Water molecules are not included.