7TPS

Crystal structure of ALPN-202 (engineered CD80 vIgD) in complex with PD-L1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	T-lymphocyte activation antigen CD80	polymer	106	12245.2	2	UniProt (P33681) Pfam (PF07686) In PDB	Homo sapiens (human)	Activation B7-1 antigen,BB1,CTLA-4 counter-receptor B7.1,B7
2	B	Programmed cell death 1 ligand 1	polymer	209	24048.2	1	UniProt (Q9NZQ7) Pfam (PF07686) Pfam (PF08205) In PDB	Homo sapiens (human)	PD-L1,PDCD1 ligand 1,Programmed death ligand 1,hPD-L1,B7 homolog 1,B7-H1
3	D	Programmed cell death 1 ligand 1	polymer	209	23968.2	1	UniProt (Q9NZQ7) Pfam (PF07686) Pfam (PF08205) In PDB	Homo sapiens (human)	PD-L1,PDCD1 ligand 1,Programmed death ligand 1,hPD-L1,B7 homolog 1,B7-H1
4	E, G, H	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	3	In PDB GlyTouCan (G21290RB)
5	F	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB
6	A, B, C, D	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
7	D	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8		water	water		18.0	53	Chemie (HOH)

Sequence modifications

A, C: 35 - 140 (UniProt: P33681)

PDB	External Database	Details
Tyr 52	His 52	engineered mutation
Glu 60	Ala 60	engineered mutation
Asp 69	Glu 69	engineered mutation
Leu 81	Met 81	engineered mutation
Met 102	Val 102	engineered mutation
Gly 105	Ala 105	engineered mutation
Gly 124	Asp 124	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	72506.7
Branched	Number of molecules	4
	Total formula weight	2282.2
Non-Polymers*	Number of molecules	7
	Total formula weight	1419.3
All*	Total formula weight	76208.2

*Water molecules are not included.