7TN2

Composite model of a Chd1-nucleosome complex in the nucleotide-free state derived from 2.3A and 2.7A Cryo-EM maps

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3	polymer	135	15271.9	2	UniProt (A0A310TTQ1) Pfam (PF00125) UniProt (by SIFTS) (P84233)	Xenopus laevis (African clawed frog)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B	polymer	122	13540.8	2	UniProt (A0A1L8FQ56) Pfam (PF00125) UniProt (by SIFTS) (P02281)	Xenopus laevis (African clawed frog)
5	I (I)	DNA Tracking Strand	polymer	226	69501.2	1		synthetic construct
6	J (J)	DNA Lagging Strand	polymer	226	70066.6	1		synthetic construct
7	K (W)	Chromo domain-containing protein 1	polymer	1159	133305.3	1	UniProt (P32657) Pfam (PF00385) Pfam (PF00176) Pfam (PF00271) Pfam (PF18196)	Saccharomyces cerevisiae (baker's yeast)	ATP-dependent helicase CHD1

Sequence modifications

A, E: 1 - 135 (UniProt: A0A310TTQ1)

PDB	External Database	Details
Ala 110	Cys 111	engineered mutation

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation
Ser 123	Ala 124	engineered mutation

D, H: 1 - 122 (UniProt: A0A1L8FQ56)

PDB	External Database	Details
Pro 7	Ala 11	conflict
Cys 53	Ser 57	engineered mutation

W: 118 - 1274 (UniProt: P32657)

PDB	External Database	Details
Gly 116	-	expression tag
Pro 117	-	expression tag

Sequence viewer

Contents of the asymmetric unit

*Water molecules are not included.