7TID
Structure of the yeast clamp loader (Replication Factor C RFC) bound to the sliding clamp (Proliferating Cell Nuclear Antigen PCNA) and primer-template DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Replication factor C subunit 1 | polymer | 861 | 95048.2 | 1 | UniProt (P38630) Pfam (PF00533) Pfam (PF00004) Pfam (PF08519) | Saccharomyces cerevisiae (baker's yeast) | Replication factor C1,Activator 1 95 kDa subunit,Cell division control protein 44 |
| 2 | B (B) | Replication factor C subunit 4 | polymer | 323 | 36201.0 | 1 | UniProt (P40339) Pfam (PF00004) Pfam (PF08542) | Saccharomyces cerevisiae (baker's yeast) | Replication factor C4,Activator 1 37 kDa subunit |
| 3 | C (C) | Replication factor C subunit 3 | polymer | 340 | 38254.5 | 1 | UniProt (P38629) Pfam (PF00004) Pfam (PF08542) | Saccharomyces cerevisiae (baker's yeast) | Replication factor C3,Activator 1 40 kDa subunit |
| 4 | D (D) | Replication factor C subunit 2 | polymer | 353 | 39794.5 | 1 | UniProt (P40348) Pfam (PF08542) Pfam (PF00004) Pfam (PF21960) | Saccharomyces cerevisiae (baker's yeast) | Replication factor C2,Activator 1 41 kDa subunit |
| 5 | E (E) | Replication factor C subunit 5 | polymer | 354 | 39993.6 | 1 | UniProt (P38251) Pfam (PF13177) Pfam (PF21960) Pfam (PF22534) | Saccharomyces cerevisiae (baker's yeast) | Replication factor C5,Activator 1 40 kDa subunit |
| 6 | F, G, H (F, G, H) | Proliferating cell nuclear antigen | polymer | 264 | 29525.7 | 3 | UniProt (P15873) Pfam (PF00705) Pfam (PF02747) | Saccharomyces cerevisiae (baker's yeast) | PCNA |
| 7 | I (I) | DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*AP*TP*GP*TP*AP*CP*TP*CP*GP*TP*AP*GP*TP*GP*TP*CP*T)-3') | polymer | 30 | 9172.9 | 1 | synthetic construct | ||
| 8 | J (J) | DNA (5'-D(*AP*GP*AP*CP*AP*CP*TP*AP*CP*GP*AP*GP*TP*AP*CP*AP*TP*A)-3') | polymer | 20 | 6136.0 | 1 | synthetic construct | ||
| 9 | K, M, O, Q (A, B, C, D) | PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER | non-polymer | 523.2 | 4 | Chemie (AGS) | |||
| 10 | L, N, P, R (A, B, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 11 | S (E) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) |
Sequence modifications
F, G, H: 1 - 258 (UniProt: P15873)
| PDB | External Database | Details |
|---|---|---|
| Gly -5 | - | expression tag |
| Pro -4 | - | expression tag |
| His -3 | - | expression tag |
| Met -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 353177.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2617.4 | |
| All* | Total formula weight | 355795.3 |
