7TEE

Cryo-EM structure of GluN1b-2B NMDAR complexed to Fab2 Non-active2-like

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Glutamate receptor ionotropic, NMDA 1	polymer	862	96944.9	2	UniProt (P35439) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus	GluN1,Glutamate [NMDA] receptor subunit zeta-1,N-methyl-D-aspartate receptor subunit NR1,NMD-R1
2	B, D (B, D)	Glutamate receptor ionotropic, NMDA 2B	polymer	883	98797.8	2	UniProt (Q00960) Pfam (PF01094) Pfam (PF10613) Pfam (PF00060)	Rattus norvegicus	GluN2B,Glutamate [NMDA] receptor subunit epsilon-2,N-methyl D-aspartate receptor subtype 2B,NMDAR2B,NR2B
3	E, G (H, M)	Fab2 heavy chain	polymer	223	23914.8	2		Mus musculus (Mouse)
4	F, H (L, N)	Fab2 light chain	polymer	213	23572.0	2		Mus musculus (Mouse)

Sequence modifications

A, C: 1 - 859 (UniProt: P35439)

PDB	External Database	Details
Ser 22	Cys 22	conflict
Gln 61	Asn 61	conflict
Asp 260	Asn 260	conflict
Gln 371	Asn 371	conflict
Gln 492	Asn 492	conflict
Gln 512	Asn 512	conflict
Gln 615	Glu 615	conflict
Ser 616	Glu 616	conflict
Ser 618	Glu 618	conflict
Thr 619	Glu 619	conflict
Gln 792	Asn 792	conflict
Cys 831	Phe 831	conflict
Ser 860	-	expression tag
Arg 861	-	expression tag
Ala 862	-	expression tag

B, D: 27 - 852 (UniProt: Q00960)

PDB	External Database	Details
Met -30	-	expression tag
Gly -29	-	expression tag
Thr -28	-	expression tag
Met -27	-	expression tag
Arg -26	-	expression tag
Leu -25	-	expression tag
Phe -24	-	expression tag
Leu -23	-	expression tag
Leu -22	-	expression tag
Ala -21	-	expression tag
Val -20	-	expression tag
Leu -19	-	expression tag
Phe -18	-	expression tag
Leu -17	-	expression tag
Phe -16	-	expression tag
Ser -15	-	expression tag
Phe -14	-	expression tag
Ala -13	-	expression tag
Arg -12	-	expression tag
Ala -11	-	expression tag
Thr -10	-	expression tag
Gly -9	-	expression tag
Trp -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
Pro -5	-	expression tag
Gln -4	-	expression tag
Phe -3	-	expression tag
Glu -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Gly 4	-	expression tag
Gly 5	-	expression tag
Gly 6	-	expression tag
Ser 7	-	expression tag
Gly 8	-	expression tag
Gly 9	-	expression tag
Ser 10	-	expression tag
Ala 11	-	expression tag
Trp 12	-	expression tag
Ser 13	-	expression tag
His 14	-	expression tag
Pro 15	-	expression tag
Gln 16	-	expression tag
Phe 17	-	expression tag
Glu 18	-	expression tag
Lys 19	-	expression tag
Gly 20	-	expression tag
Ala 21	-	expression tag
Leu 22	-	expression tag
Val 23	-	expression tag
Pro 24	-	expression tag
Arg 25	-	expression tag
Gly 26	-	expression tag
Asp 348	Asn 348	conflict
Cys 557	Asp 557	conflict
Ser 588	Cys 588	conflict
Val 600	Phe 600	conflict
Ser 838	Cys 838	conflict
Ser 849	Cys 849	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	486459.0
All*	Total formula weight	486459.0

*Water molecules are not included.