7TCW

Methanobactin biosynthetic protein complex of MbnB and MbnC from Methylosinus trichosporium OB3b, H210S mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Methanobactin biosynthesis cassette protein MbnB	polymer	268	31271.4	2	UniProt (A0A2D2D5M1) Pfam (PF05114) In PDB	Methylosinus trichosporium OB3b
2	C, D	Methanobactin biosynthesis cassette protein MbnC	polymer	195	22237.4	2	UniProt (A0A2D2CY73) In PDB	Methylosinus trichosporium OB3b
3	A, B	FE (III) ION	non-polymer		55.8	4	Chemie (FE)
4	A, B	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
5		water	water		18.0	98	Chemie (HOH)

Sequence modifications

A, B: 1 - 268 (UniProt: A0A2D2D5M1)

PDB	External Database	Details
Ala 67	Glu 67	engineered mutation
Ala 69	Glu 69	engineered mutation
Ala 70	Lys 70	engineered mutation
Gly 110	Arg 110	conflict
Ser 210	His 210	engineered mutation

C, D: 1 - 195 (UniProt: A0A2D2CY73)

PDB	External Database	Details
Ala 162	Glu 162	engineered mutation
Ala 164	Glu 164	engineered mutation
Ala 165	Lys 165	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	107017.6
Non-Polymers*	Number of molecules	8
	Total formula weight	607.6
All*	Total formula weight	107625.3

*Water molecules are not included.