7TCO
Cryo-EM structure of CH235.12 in complex with HIV-1 Env trimer CH505TF.N279K.G458Y.SOSIP.664 with high-mannose glycans
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I (A, E, M) | Envelope glycoprotein gp120 | polymer | 461 | 52019.3 | 3 | UniProt (M4M0W3) | Human immunodeficiency virus 1 | Env polyprotein |
| 2 | B, F, J (B, F, N) | Glycoprotein 41 | polymer | 160 | 18146.7 | 3 | UniProt (Q2N0S5) | Human immunodeficiency virus 1 | Transmembrane protein gp41, TM, gp41 |
| 3 | C, G, K (C, G, O) | CH235.12 Fab Heavy Chain | polymer | 225 | 24319.4 | 3 | Homo sapiens (Human) | ||
| 4 | D, H, L (D, H, P) | CH235.12 Fab Light Chain | polymer | 213 | 23451.0 | 3 | Homo sapiens (Human) | ||
| 5 | M, Q, U (I, Q, V) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 3 | In PDB GlyTouCan (G15407YE) | |||
| 6 | N, R, V (J, R, W) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 7 | O, S, W (K, S, X) | alpha-D-mannopyranose-(1-6)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 3 | In PDB GlyTouCan (G34442SS) | |||
| 8 | P, T, X (L, T, Y) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 3 | In PDB GlyTouCan (G81315DD) | |||
| 9 | AA, BA, CA, DA, EA... (A, E, M) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 12 | Chemie (NAG) |
Sequence modifications
A, E, M: 35 - 503 (UniProt: M4M0W3)
B, F, N: 511 - 664 (UniProt: Q2N0S5)
| PDB | External Database | Details |
|---|---|---|
| Glu 32 | - | expression tag |
| Asn 33 | - | expression tag |
| Leu 34 | - | expression tag |
| Lys 64 | Glu 60 | conflict |
| Trp 316 | Ala 301 | conflict |
| Tyr 458 | Gly 443 | conflict |
| Lys 488 | Glu 473 | conflict |
| Ile 489 | Val 474 | conflict |
| Glu 490 | Lys 475 | conflict |
| Arg 498 | Asn 483 | conflict |
| Cys 499 | Ala 484 | conflict |
| Lys 500 | Arg 485 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Gly 505 | - | expression tag |
| Arg 506 | - | expression tag |
| Arg 507 | - | expression tag |
| Arg 508 | - | expression tag |
| Arg 509 | - | expression tag |
| Arg 510 | - | expression tag |
| Pro 559 | Ile 556 | conflict |
| Cys 605 | Thr 602 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 353809.2 | |
| Branched | Number of molecules | 12 |
| Total formula weight | 8497.8 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 2654.5 | |
| All* | Total formula weight | 364961.5 |
