7TAW

Cryo-EM structure of the Csy-AcrIF24-promoter DNA dimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, a	CRISPR-associated protein Csy1	polymer	434	49194.2	2	UniProt (Q02ML9) Pfam (PF09611) In PDB	Pseudomonas
2	B, b	CRISPR type I-F/YPEST-associated protein Csy2	polymer	327	36244.1	2	UniProt (B3G161) Pfam (PF09614) UniProt (by SIFTS) (Q02MM0) In PDB	Pseudomonas	Type I-F CRISPR-associated protein Csy2
3	C, c	CRISPR-associated endonuclease Cas6/Csy4	polymer	187	21427.5	2	UniProt (Q02MM2) Pfam (PF09618) In PDB	Pseudomonas
4	D, E, F, G, H...	CRISPR type I-F/YPEST-associated protein Csy3	polymer	360	39778.6	12	UniProt (A0A444M080) Pfam (PF09615) UniProt (by SIFTS) (Q02MM1) In PDB	Pseudomonas
5	J, K	AcrIF24	polymer	228	24995.3	2		Pseudomonas
6	M, m	RNA (61-MER)	polymer	61	19538.6	2	GenBank (313291946)	Pseudomonas
7	Y	DNA (5'-D(P*AP*TP*AP*GP*CP*TP*CP*GP*AP*TP*TP*CP*GP*AP*GP*CP*TP*AP*A)-3')	polymer	19	5828.8	1		Pseudomonas
8	X	DNA (5'-D(P*TP*TP*AP*GP*CP*TP*CP*GP*AP*AP*TP*CP*GP*AP*GP*CP*TP*AP*T)-3')	polymer	19	5819.8	1		Pseudomonas

Sequence modifications

D, E, F, G, H, I, d, e, f, g, h, i: 21 - 361 (UniProt: A0A444M080)

PDB	External Database	Details
Met 2	-	initiating methionine
Lys 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Glu 12	-	expression tag
Asn 13	-	expression tag
Leu 14	-	expression tag
Tyr 15	-	expression tag
Phe 16	-	expression tag
Gln 17	-	expression tag
Ser 18	-	expression tag
Asn 19	-	expression tag
Ala 20	-	expression tag

M, m: 1 - 61 (GenBank: 313291946)

PDB	External Database	Details
A 41	G 1458	conflict
A 53	G 1446	conflict

Contents of the asymmetric unit

Polymers	Number of chains	24
	Total formula weight	791790.9
All*	Total formula weight	791790.9

*Water molecules are not included.