7T9I
Native human TSH bound to human Thyrotropin receptor in complex with miniGs399 (composite structure)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Glycoprotein hormones alpha chain | polymer | 92 | 10217.8 | 1 | UniProt (P01215) Pfam (PF00236) | Homo sapiens (human) | Anterior pituitary glycoprotein hormones common subunit alpha,Choriogonadotropin alpha chain,Chorionic gonadotrophin subunit alpha,CG-alpha,Follicle-stimulating hormone alpha chain,FSH-alpha,Follitropin alpha chain,Luteinizing hormone alpha chain,LSH-alpha,Lutropin alpha chain,Thyroid-stimulating hormone alpha chain,TSH-alpha,Thyrotropin alpha chain |
| 2 | B (B) | Thyrotropin subunit beta | polymer | 112 | 12916.0 | 1 | UniProt (P01222) Pfam (PF00007) | Homo sapiens (human) | Thyroid-stimulating hormone subunit beta,TSH-B,TSH-beta,Thyrotropin beta chain |
| 3 | C (N) | Nanobody 35 | polymer | 142 | 15398.1 | 1 | Lama glama | ||
| 4 | D (R) | Thyrotropin receptor | polymer | 702 | 79742.4 | 1 | UniProt (A0A0A0MTJ0) Pfam (PF13306) Pfam (PF00001) UniProt (by SIFTS) (P16473) | Homo sapiens (human) | |
| 5 | E (X) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 261 | 30137.0 | 1 | UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-stimulating G alpha protein |
| 6 | F (Y) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 370 | 40786.6 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 7 | G (Z) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 8 | H (C) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 9 | I, J, K, L, M... (A, B, R) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 6 | Chemie (NAG) |
Sequence modifications
R: 22 - 764 (UniProt: A0A0A0MTJ0)
X: 204 - 394 (UniProt: P63092)
Y: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 13 | - | expression tag |
| Tyr 14 | - | expression tag |
| Lys 15 | - | expression tag |
| Asp 16 | - | expression tag |
| Asp 17 | - | expression tag |
| Asp 18 | - | expression tag |
| Asp 19 | - | expression tag |
| Gly 20 | - | expression tag |
| Thr 21 | - | expression tag |
| - | Ala 317 | deletion |
| - | Leu 318 | deletion |
| - | Asn 319 | deletion |
| - | Ser 320 | deletion |
| - | Pro 321 | deletion |
| - | Leu 322 | deletion |
| - | His 323 | deletion |
| - | Gln 324 | deletion |
| - | Glu 325 | deletion |
| - | Tyr 326 | deletion |
| - | Glu 327 | deletion |
| - | Glu 328 | deletion |
| - | Asn 329 | deletion |
| - | Leu 330 | deletion |
| - | Gly 331 | deletion |
| - | Asp 332 | deletion |
| - | Ser 333 | deletion |
| - | Ile 334 | deletion |
| - | Val 335 | deletion |
| - | Gly 336 | deletion |
| - | Tyr 337 | deletion |
| - | Lys 338 | deletion |
| - | Glu 339 | deletion |
| - | Lys 340 | deletion |
| - | Ser 341 | deletion |
| - | Lys 342 | deletion |
| - | Phe 343 | deletion |
| - | Gln 344 | deletion |
| - | Asp 345 | deletion |
| - | Thr 346 | deletion |
| - | His 347 | deletion |
| - | Asn 348 | deletion |
| - | Asn 349 | deletion |
| - | Ala 350 | deletion |
| - | His 351 | deletion |
| - | Tyr 352 | deletion |
| - | Tyr 353 | deletion |
| - | Val 354 | deletion |
| - | Phe 355 | deletion |
| - | Phe 356 | deletion |
| - | Glu 357 | deletion |
| - | Glu 358 | deletion |
| - | Gln 359 | deletion |
| - | Glu 360 | deletion |
| - | Asp 361 | deletion |
| - | Glu 362 | deletion |
| - | Ile 363 | deletion |
| - | Ile 364 | deletion |
| - | Gly 365 | deletion |
| - | Phe 366 | deletion |
| PDB | External Database | Details |
|---|---|---|
| Gly -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Glu -3 | - | expression tag |
| Val -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Phe 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 2 | - | expression tag |
| Pro 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Gly 5 | - | expression tag |
| Asn 6 | - | expression tag |
| Ser 7 | - | expression tag |
| Lys 8 | - | expression tag |
| Thr 9 | - | expression tag |
| Glu 10 | - | expression tag |
| Asp 11 | - | expression tag |
| Gln 12 | - | expression tag |
| Arg 13 | - | expression tag |
| Asn 14 | - | expression tag |
| Glu 15 | - | expression tag |
| Glu 16 | - | expression tag |
| Lys 17 | - | expression tag |
| Ala 18 | - | expression tag |
| Gln 19 | - | expression tag |
| Arg 20 | - | expression tag |
| Glu 21 | - | expression tag |
| Ala 22 | - | expression tag |
| Asn 23 | - | expression tag |
| Lys 24 | - | expression tag |
| Lys 25 | - | expression tag |
| Ile 26 | - | expression tag |
| Glu 27 | - | expression tag |
| Lys 28 | - | expression tag |
| Gln 29 | - | expression tag |
| Leu 30 | - | expression tag |
| Gln 31 | - | expression tag |
| Lys 32 | - | expression tag |
| Asp 33 | - | expression tag |
| Lys 34 | - | expression tag |
| Gln 35 | - | expression tag |
| Val 36 | - | expression tag |
| Tyr 37 | - | expression tag |
| Arg 38 | - | expression tag |
| Ala 39 | - | expression tag |
| Thr 40 | - | expression tag |
| His 41 | - | expression tag |
| Arg 42 | - | expression tag |
| Leu 43 | - | expression tag |
| Leu 44 | - | expression tag |
| Leu 45 | - | expression tag |
| Leu 46 | - | expression tag |
| Gly 47 | - | expression tag |
| Ala 48 | - | expression tag |
| Asp 49 | - | expression tag |
| Asn 50 | - | expression tag |
| Ser 51 | - | expression tag |
| Gly 52 | - | expression tag |
| Lys 53 | - | expression tag |
| Ser 54 | - | expression tag |
| Thr 55 | - | expression tag |
| Ile 56 | - | expression tag |
| Val 57 | - | expression tag |
| Lys 58 | - | expression tag |
| Gln 59 | - | expression tag |
| Met 60 | - | expression tag |
| Arg 61 | - | expression tag |
| Ile 193 | - | expression tag |
| Leu 194 | - | expression tag |
| His 195 | - | expression tag |
| Gly 196 | - | expression tag |
| Gly 197 | - | expression tag |
| Ser 198 | - | expression tag |
| Gly 199 | - | expression tag |
| Gly 200 | - | expression tag |
| Ser 201 | - | expression tag |
| Gly 202 | - | expression tag |
| Gly 203 | - | expression tag |
| Asp 249 | Ala 249 | conflict |
| Asp 252 | Ser 252 | conflict |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Leu 308 | Ile 308 | conflict |
| Ala 372 | Ile 372 | conflict |
| Ile 375 | Val 375 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met -29 | - | initiating methionine |
| His -28 | - | expression tag |
| His -27 | - | expression tag |
| His -26 | - | expression tag |
| His -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| Leu -22 | - | expression tag |
| Glu -21 | - | expression tag |
| Val -20 | - | expression tag |
| Leu -19 | - | expression tag |
| Phe -18 | - | expression tag |
| Gln -17 | - | expression tag |
| Gly -16 | - | expression tag |
| Pro -15 | - | expression tag |
| Glu -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Gln -12 | - | expression tag |
| Val -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Pro -9 | - | expression tag |
| Arg -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Ile -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Lys -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 197058.9 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 424.4 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1327.2 | |
| All* | Total formula weight | 198810.6 |
