7T57

Cryo-EM structure of PCAT1 in the inward-facing narrow conformation under ATP turnover condition

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	ABC-type bacteriocin transporter	polymer	730	81180.8	2	UniProt (A3DCU1) Pfam (PF03412) Pfam (PF00664) Pfam (PF00005)	Acetivibrio thermocellus
2	B, D (C, D)	PCAT1 peptide substrate	polymer	93	10217.9	2	UniProt (A3DCU2)	Acetivibrio thermocellus
3	E, G (A, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
4	F, H (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

A, B: 1 - 727 (UniProt: A3DCU1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

C, D: 1 - 90 (UniProt: A3DCU2)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	182797.3
Non-Polymers*	Number of molecules	4
	Total formula weight	1063.0
All*	Total formula weight	183860.3

*Water molecules are not included.