7T4R

CryoEM structure of the HCMV Pentamer gH/gL/UL128/UL130/UL131A in complex with THBD and neutralizing fabs MSL-109 and 13H11

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Thrombomodulin	polymer	527	55128.4	1	UniProt (P07204) Pfam (PF00059) Pfam (PF25444) Pfam (PF14670) Pfam (PF12662)	Homo sapiens (human)	TM,Fetomodulin
2	B, K (B, K)	Envelope glycoprotein H	polymer	767	87311.3	2	UniProt (F5H9T3) Pfam (PF02489) Pfam (PF17488) UniProt (by SIFTS) (Q6SW67)	Human betaherpesvirus 5	gH
3	C, L (C, L)	Envelope glycoprotein L	polymer	278	30846.5	2	UniProt (Q71DN9) Pfam (PF01801) UniProt (by SIFTS) (F5HCH8)	Human betaherpesvirus 5	gL
4	D, M (D, M)	Envelope protein UL128	polymer	171	19746.0	2	UniProt (Q38LY2) UniProt (by SIFTS) (V9LLX6)	Human betaherpesvirus 5	UL128
5	E, N (E, N)	Envelope glycoprotein UL130	polymer	254	28866.8	2	UniProt (Q38M07) Pfam (PF11668) UniProt (by SIFTS) (F5HCP3)	Human betaherpesvirus 5	UL130
6	F, O (F, O)	Envelope protein UL131A	polymer	129	15011.0	2	UniProt (Q8AZ45) UniProt (by SIFTS) (F5HET4)	Human betaherpesvirus 5	Protein UL131A,UL131A
7	G, P (G, P)	Fab 13H11 heavy chain	polymer	250	26600.1	2		Homo sapiens
8	H, Q (H, Q)	Fab 13H11 light chain	polymer	237	25780.0	2		Homo sapiens
9	I, R (I, R)	Fab MSL-109 light chain	polymer	257	28355.8	2		Homo sapiens
10	J, S (J, S)	Fab MSL-109 heavy chain	polymer	257	27547.8	2		Homo sapiens
11	AA, BA, CA, DA, EA... (K, N, O, B, C...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	14	Chemie (NAG)

Sequence modifications

A: 1 - 516 (UniProt: P07204)

PDB	External Database	Details
Gly 517	-	expression tag
Asn 518	-	expression tag
Ser 519	-	expression tag
Asp 520	-	expression tag
Tyr 521	-	expression tag
Lys 522	-	expression tag
Asp 523	-	expression tag
Asp 524	-	expression tag
Asp 525	-	expression tag
Asp 526	-	expression tag
Lys 527	-	expression tag

B, K: 1 - 715 (UniProt: F5H9T3)

PDB	External Database	Details
Gly 716	-	expression tag
Thr 717	-	expression tag
Lys 718	-	expression tag
Leu 719	-	expression tag
Gly 720	-	expression tag
Pro 721	-	expression tag
Glu 722	-	expression tag
Gln 723	-	expression tag
Lys 724	-	expression tag
Leu 725	-	expression tag
Ile 726	-	expression tag
Ser 727	-	expression tag
Glu 728	-	expression tag
Glu 729	-	expression tag
Asp 730	-	expression tag
Leu 731	-	expression tag
Asn 732	-	expression tag
Ser 733	-	expression tag
Ala 734	-	expression tag
Val 735	-	expression tag
Asp 736	-	expression tag
Gly 737	-	expression tag
Ser 738	-	expression tag
Gly 739	-	expression tag
Leu 740	-	expression tag
Asn 741	-	expression tag
Asp 742	-	expression tag
Ile 743	-	expression tag
Phe 744	-	expression tag
Glu 745	-	expression tag
Ala 746	-	expression tag
Gln 747	-	expression tag
Lys 748	-	expression tag
Ile 749	-	expression tag
Glu 750	-	expression tag
Trp 751	-	expression tag
His 752	-	expression tag
Glu 753	-	expression tag
Asn 754	-	expression tag
Leu 755	-	expression tag
Tyr 756	-	expression tag
Phe 757	-	expression tag
Gln 758	-	expression tag
Gly 759	-	expression tag
His 760	-	expression tag
His 761	-	expression tag
His 762	-	expression tag
His 763	-	expression tag
His 764	-	expression tag
His 765	-	expression tag
His 766	-	expression tag
His 767	-	expression tag

E, N: 1 - 214 (UniProt: Q38M07)

PDB	External Database	Details
Gly 215	-	expression tag
Ser 216	-	expression tag
Glu 217	-	expression tag
Asn 218	-	expression tag
Leu 219	-	expression tag
Tyr 220	-	expression tag
Phe 221	-	expression tag
Gln 222	-	expression tag
Gly 223	-	expression tag
Ser 224	-	expression tag
Ala 225	-	expression tag
Trp 226	-	expression tag
Ser 227	-	expression tag
His 228	-	expression tag
Pro 229	-	expression tag
Gln 230	-	expression tag
Phe 231	-	expression tag
Glu 232	-	expression tag
Lys 233	-	expression tag
Gly 234	-	expression tag
Gly 235	-	expression tag
Gly 236	-	expression tag
Ser 237	-	expression tag
Gly 238	-	expression tag
Gly 239	-	expression tag
Gly 240	-	expression tag
Ser 241	-	expression tag
Gly 242	-	expression tag
Gly 243	-	expression tag
Gly 244	-	expression tag
Ser 245	-	expression tag
Ala 246	-	expression tag
Trp 247	-	expression tag
Ser 248	-	expression tag
His 249	-	expression tag
Pro 250	-	expression tag
Gln 251	-	expression tag
Phe 252	-	expression tag
Glu 253	-	expression tag
Lys 254	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	19
	Total formula weight	635259.1
Non-Polymers*	Number of molecules	14
	Total formula weight	3096.9
All*	Total formula weight	638356.0

*Water molecules are not included.