7T3L
Cryo-EM structure of Csy-AcrIF24-DNA dimer
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, a | CRISPR-associated protein Csy1 | polymer | 434 | 49194.2 | 2 | UniProt (Q02ML9) Pfam (PF09611) In PDB | Pseudomonas | |
| 2 | B, b | CRISPR type I-F/YPEST-associated protein Csy2 | polymer | 327 | 36244.1 | 2 | UniProt (B3G161) Pfam (PF09614) UniProt (by SIFTS) (Q02MM0) In PDB | Pseudomonas | Type I-F CRISPR-associated protein Csy2 |
| 3 | C, c | CRISPR-associated endonuclease Cas6/Csy4 | polymer | 187 | 21427.5 | 2 | UniProt (Q02MM2) Pfam (PF09618) In PDB | Pseudomonas | |
| 4 | D, E, F, G, H... | CRISPR type I-F/YPEST-associated protein Csy3 | polymer | 360 | 39778.6 | 12 | UniProt (A0A444M080) Pfam (PF09615) UniProt (by SIFTS) (Q02MM1) In PDB | Pseudomonas | |
| 5 | J, K | AcrIF24 | polymer | 228 | 24995.3 | 2 | UniProt (A0A8G3G219) In PDB | Pseudomonas | |
| 6 | M, m | RNA (61-MER) | polymer | 61 | 19538.6 | 2 | Pseudomonas | ||
| 7 | O, R, S | DNA (5'-D(P*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*GP*G)-3') | polymer | 21 | 6868.4 | 3 | Escherichia | ||
| 8 | P, Q, T | DNA (5'-D(P*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*CP*C)-3') | polymer | 21 | 6027.9 | 3 | Escherichia |
Sequence modifications
D, E, F, G, H, I, d, e, f, g, h, i: 21 - 361 (UniProt: A0A444M080)
J, K: 1 - 228 (UniProt: A0A8G3G219)
| PDB | External Database | Details |
|---|---|---|
| Met 2 | - | initiating methionine |
| Lys 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Ser 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| His 10 | - | expression tag |
| His 11 | - | expression tag |
| Glu 12 | - | expression tag |
| Asn 13 | - | expression tag |
| Leu 14 | - | expression tag |
| Tyr 15 | - | expression tag |
| Phe 16 | - | expression tag |
| Gln 17 | - | expression tag |
| Ser 18 | - | expression tag |
| Asn 19 | - | expression tag |
| Ala 20 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gln 27 | Arg 27 | conflict |
| His 68 | Asp 68 | conflict |
| Ala 97 | Gly 97 | conflict |
| Leu 112 | Val 112 | conflict |
| Val 133 | Ile 133 | conflict |
| Lys 225 | Met 225 | conflict |
| Ala 227 | Thr 227 | conflict |
| Ser 228 | Asn 228 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 28 |
| Total formula weight | 818831.0 | |
| All* | Total formula weight | 818831.0 |
