7T3J
Cryo-EM structure of Csy-AcrIF24
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | CRISPR-associated protein Csy1 | polymer | 434 | 49194.2 | 1 | UniProt (Q02ML9) | Pseudomonas | |
| 2 | B (B) | CRISPR type I-F/YPEST-associated protein Csy2 | polymer | 327 | 36244.1 | 1 | UniProt (B3G161) UniProt (by SIFTS) (Q02MM0) | Pseudomonas | Type I-F CRISPR-associated protein Csy2 |
| 3 | C (C) | CRISPR-associated endonuclease Cas6/Csy4 | polymer | 187 | 21427.5 | 1 | UniProt (Q02MM2) | Pseudomonas | |
| 4 | D, E, F, G, H... (D, E, F, G, H...) | CRISPR type I-F/YPEST-associated protein Csy3 | polymer | 360 | 39778.6 | 6 | UniProt (A0A444M080) UniProt (by SIFTS) (Q02MM1) | Pseudomonas | |
| 5 | J, K (J, K) | AcrIF24 | polymer | 228 | 24995.3 | 2 | Pseudomonas | ||
| 6 | L (M) | RNA (61-MER) | polymer | 61 | 19538.6 | 1 | Pseudomonas |
Sequence modifications
D, E, F, G, H, I: 21 - 361 (UniProt: A0A444M080)
| PDB | External Database | Details |
|---|---|---|
| Met 2 | - | initiating methionine |
| Lys 3 | - | expression tag |
| Ser 4 | - | expression tag |
| Ser 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| His 9 | - | expression tag |
| His 10 | - | expression tag |
| His 11 | - | expression tag |
| Glu 12 | - | expression tag |
| Asn 13 | - | expression tag |
| Leu 14 | - | expression tag |
| Tyr 15 | - | expression tag |
| Phe 16 | - | expression tag |
| Gln 17 | - | expression tag |
| Ser 18 | - | expression tag |
| Asn 19 | - | expression tag |
| Ala 20 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 415066.4 | |
| All* | Total formula weight | 415066.4 |
