7T2H
CryoEM structure of mu-opioid receptor - Gi protein complex bound to lofentanil (LFT)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 344 | 37671.1 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (E) | scFv16 | polymer | 259 | 27784.9 | 1 | Mus musculus (mouse) | ||
| 5 | E (D) | Mu-type opioid receptor | polymer | 356 | 39995.1 | 1 | UniProt (P42866) Pfam (PF00001) | Mus musculus (house mouse) | M-OR-1,MOR-1 |
| 6 | F (D) | Lofentanil | non-polymer | 408.5 | 1 | Chemie (EID) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
D: 3 - 358 (UniProt: P42866)
| PDB | External Database | Details |
|---|---|---|
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Glu 46 | - | insertion |
| Asn 47 | - | insertion |
| Leu 48 | - | insertion |
| Tyr 49 | - | insertion |
| Phe 50 | - | insertion |
| Gln 51 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 153727.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 408.5 | |
| All* | Total formula weight | 154135.8 |
