7T22

E. coli DnaB bound to three DnaG C-terminal domains, ssDNA, ADP and AlF4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Replicative DNA helicase	polymer	471	52406.9	6	UniProt (P0ACB0) Pfam (PF00772) Pfam (PF03796) In PDB	Escherichia coli K-12
2	G, H, I	DNA primase	polymer	148	16664.9	3	UniProt (P0ABS5) Pfam (PF08278) In PDB	Escherichia coli K-12
3	M	DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')	polymer	20	6038.9	1		Escherichia coli
4	B, C, D, E, F	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	5	Chemie (ADP)
5	B, C, D, E, F	TETRAFLUOROALUMINATE ION	non-polymer		103.0	5	Chemie (ALF)
6	B, C, D, E, F	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)

Sequence modifications

A, B, C, D, E, F: 1 - 471 (UniProt: P0ACB0)

PDB	External Database	Details
Cys 103	Phe 103	engineered mutation

G, H, I: 434 - 581 (UniProt: P0ABS5)

PDB	External Database	Details
Cys 568	Arg 568	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	370475.2
Non-Polymers*	Number of molecules	15
	Total formula weight	2772.4
All*	Total formula weight	373247.6

*Water molecules are not included.